A PARVO-LIKE VIRUS PERSISTENTLY INFECTING A C6/36 CLONE OF AEDES/ALBOPICTUS MOSQUITO CELL-LINE AND PATHOGENIC FOR AEDES-AEGYPTI LARVAE
A PARVO-LIKE VIRUS PERSISTENTLY INFECTING A C6/36 CLONE OF AEDES/ALBOPICTUS MOSQUITO CELL-LINE AND PATHOGENIC FOR AEDES-AEGYPTI LARVAE
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DOI：
10.1016/0168-1702(93)90052-o
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发表时间：
1993-08-01
期刊：
影响因子：
5
通讯作者：
CORNET, M
中科院分区：
文献类型：
--
作者：
JOUSSET, FX;BARREAU, C;CORNET, M
我们已经分离和部分特点，从一个明显健康的C6/36亚克隆白纹伊蚊细胞系一个小的二十面体无包膜DNA病毒，指定AaPV。该病毒被证明对埃及伊蚊新生幼虫具有高致病性。病毒感染在细胞培养物中持续4年以上，无任何细胞病变效应。用AaPV感染乳鼠、黑腹果蝇（Drosophila melanogaster）成虫和斜纹夜蛾（Spodoptera littoralis）幼虫的尝试均未成功。类似地，AaPV未能在脊椎动物和果蝇细胞系中复制。直径约22 nm的病毒粒子具有1.43 g/cm 3的浮力密度，并且含有分子量为53、41和40 kDa的三种衣壳多肽。对病毒基因组的初步研究表明存在单链DNA。根据其生物物理和生化特性，该病毒似乎与细小病毒科中的浓核病毒属相关，但与该属的其他成员缺乏血清学关系。
We have isolated and partially characterized from an apparently healthy C6/36 subclone of Aedes albopictus cell line a small icosahedral non-enveloped DNA virus, designated AaPV. This virus proved to be highly pathogenic for Aedes aegypti neonate larvae. Viral infection persisted for over 4 years in the cell culture without any cytopathic effect. Attempts to infect suckling mice, Drosophila melanogaster adults and Spodoptera littoralis larvae with AaPV were unsuccessful. Similarly, the AaPV failed to replicate in vertebrate and Drosophila cell lines. Virions, about 22 nm in diameter, had a buoyant density of 1.43 g/cm3 and contained three capsid polypeptides with molecular weights of 53, 41 and 40 kDa. A preliminary study of the viral genome indicated the presence of single-stranded DNA. By its biophysical and biochemical properties, this virus appears to be related to the genus Densovirus within the family Parvoviridae, but lacks serological relationships with the other members of this genus.