Slit-scan flow cytometry of mammalian chromosomes.
Slit-scan flow cytometry of mammalian chromosomes.
复制标题
哺乳动物染色体的狭缝扫描流式细胞术。
DOI：
10.1177/27.1.374608
复制
发表时间：
1979
期刊：
影响因子：
--
通讯作者：
M. Van Dilla
中科院分区：
文献类型：
--
作者：
J. Gray;D. Peters;J. Merrill;R. Martin;M. Van Dilla
A flow cytometer has been constructed which measures total fluorescence and the distribution of fluorescence along isolated, stained mammalian chromosomes. In this device, chromosomes flow lengthwise at 4 m/sec through a 1-micrometer thick laser beam. The fluorescence from each chromosome is recorded at 10 nsec intervals; the sequence of recorded values represents the distribution of fluorescence along the chromosome and is stored in the memory of a waveform recorder. The total fluorescence of each chromosome is also measured and recorded. Preliminary studies show that doublets of 1.83 micrometers diameter microspheres flow with their long axes parallel to the direction of flow and that the two microspheres are resolved in the slit-scan profile. Ethidium bromide stained Muntjac and Chinese hamster chromosomes have also been slit-scanned. Centromeres were resolved in many of the Nos. 1 and 2 Chinese hamster chromosomes and the Nos. 1 and X + 3 Muntjac chromosomes.
