Association between endotoxemia and histological features of nonalcoholic fatty liver disease.
Association between endotoxemia and histological features of nonalcoholic fatty liver disease.
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DOI：
10.3748/wjg.v23.i4.712
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发表时间：
2017-01-28
影响因子：
4.3
通讯作者：
Tanaka E
中科院分区：
文献类型：
--
作者：
Kitabatake H;Tanaka N;Fujimori N;Komatsu M;Okubo A;Kakegawa K;Kimura T;Sugiura A;Yamazaki T;Shibata S;Ichikawa Y;Joshita S;Umemura T;Matsumoto A;Koinuma M;Sano K;Aoyama T;Tanaka E
评估内毒素血症的替代生物标志物是否与非酒精性脂肪性肝病（NAFLD）的组织学特征相关。126例接受经皮肝活检的NAFLD患者入组。采用酶联免疫吸附试验测定肝活检时血清脂多糖（LPS）结合蛋白（LBP）和抗内毒素核心免疫球蛋白G（EndoCab IgG）抗体浓度，以检查生物标志物水平与组织学评分之间的关系。与非酒精性脂肪肝（NAFL）患者相比，非酒精性脂肪性肝炎（NASH）患者血清LBP浓度显著升高，并与脂肪变性（r = 0.38，P < 0.0001）和气球样变评分（r = 0.23，P = 0.01）相关，但与小叶炎症或纤维化的严重程度无关。多元线性回归分析显示LBP与脂肪变性评分、循环C反应蛋白、天冬氨酸转氨酶和纤维蛋白原水平相关。NASH和NAFL患者之间的血清EndoCab IgG浓度相当。EndoCab IgG与组织学结果之间未检测到有意义的相关性。LBP/EndoCab IgG与小叶炎症或纤维化无关。需要更准确的LPS生物标志物来严格评估内毒素血症对传统NASH的贡献。
To assess whether surrogate biomarkers of endotoxemia were correlated with the histological features of nonalcoholic fatty liver disease (NAFLD). One hundred twenty-six NAFLD patients who had undergone percutaneous liver biopsy were enrolled. Serum lipopolysaccharide (LPS)-binding protein (LBP) and anti-endotoxin core immunoglobulin G (EndoCab IgG) antibody concentrations at the time of liver biopsy were measured using the enzyme-linked immunosorbent assays to examine for relationships between biomarker levels and histological scores. Serum LBP concentration was significantly increased in nonalcoholic steatohepatitis (NASH) patients as compared with nonalcoholic fatty liver (NAFL) subjects and was correlated with steatosis (r = 0.38, P < 0.0001) and ballooning scores (r = 0.23, P = 0.01), but not with the severity of lobular inflammation or fibrosis. Multivariate linear regression analysis revealed that LBP was associated with steatosis score and circulating C-reactive protein, aspartate aminotransferase, and fibrinogen levels. Serum EndoCab IgG concentration was comparable between NASH and NAFL patients. No meaningful correlations were detected between EndoCab IgG and histological findings. LBP/EndoCab IgG were not correlated with lobular inflammation or fibrosis. More accurate LPS biomarkers are required to stringently assess the contribution of endotoxemia to conventional NASH.