Design and synthesis of new cationic water-soluble pyrene containing dendrons for DNA sensory applications
Design and synthesis of new cationic water-soluble pyrene containing dendrons for DNA sensory applications
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DOI：
10.1002/pola.25031
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发表时间：
2012-01
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影响因子：
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通讯作者：
Ching-Yi Chen;Yumiko Ito;Yu‐Cheng Chiu;W. Wu;Tomoya Higashihara;M. Ueda;Wen‐Chang Chen
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文献类型：
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作者：
Ching-Yi Chen;Yumiko Ito;Yu‐Cheng Chiu;W. Wu;Tomoya Higashihara;M. Ueda;Wen‐Chang Chen
合成并表征了一系列新型水溶性阳离子芘树枝化衍生物G1、G2和G3。这些新的树枝状分子被设计成在树枝状分子的外围具有季铵化的氨基部分，用于DNA检测，并且用芘作为荧光探针进行官能化。水溶液中质粒DNA（pDNA）与阳离子荷电树枝状分子之间的静电相互作用导致芘的光物理性质发生变化，这可以在紫外可见光谱和荧光光谱中表现出来。芘树突表现出高和快速的荧光响应后，加入pDNA，这是强烈依赖于树突的大小和疏水性。© 2011 Wiley Periodicals，Inc. J Polym Sci Part A：Polym Chem，2012
A series of new water-soluble cationic pyrene-dendron derivatives, G1, G2, and G3, was successfully synthesized and characterized. These new dendrons were designed with the quaternized amino moieties at the periphery of the dendrons for DNA detection and functionalized with pyrene as a fluorescent probe. The electrostatic interactions between the plasmid DNA (pDNA) and cationic charged dendrons in an aqueous solution resulted in a change in the photophysical properties of pyrene, which could be shown in the UV-vis and fluorescence spectra. Pyrene dendrons showed a high and rapid fluorescence response upon the addition of pDNA, which was strongly dependent on the size and hydrophobicity of the dendrons. © 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2012