Sox11 Balances Dendritic Morphogenesis with Neuronal Migration in the Developing Cerebral Cortex
Sox11 Balances Dendritic Morphogenesis with Neuronal Migration in the Developing Cerebral Cortex
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DOI：
10.1523/jneurosci.3250-15.2016
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发表时间：
2016-05-25
影响因子：
5.3
通讯作者：
Kawasaki, Hiroshi
中科院分区：
文献类型：
--
作者：
Hoshiba, Yoshio;Toda, Tomohisa;Kawasaki, Hiroshi
树突形态发生的促进和抑制之间的协调机制对大脑皮层的发育至关重要。虽然以前的研究已经揭示了重要的转录因子，促进树突状形态发生在发展过程中，那些抑制树突状形态发生仍然是未知的。在这里，我们发现，在树突状形态发生的转录因子Sox 11的表达水平显着下降。我们的损失和获得的功能的研究，使用出生后的电穿孔和在子宫内的电穿孔表明，Sox 11是必要的和足够的抑制树突状形态发生的兴奋性神经元在小鼠大脑皮层发育过程中。有趣的是，我们发现Sox11表达的早熟抑制导致神经突的早熟分支和神经元迁移缺陷。我们还发现，径向迁移的结束诱导Sox11表达的减少。这些研究结果表明，抑制树突状形态发生的Sox11在径向迁移是至关重要的大脑皮层的形成。
The coordinated mechanisms balancing promotion and suppression of dendritic morphogenesis are crucial for the development of the cerebral cortex. Although previous studies have revealed important transcription factors that promote dendritic morphogenesis during development, those that suppress dendritic morphogenesis are still largely unknown. Here we found that the expression levels of the transcription factor Sox11 decreased dramatically during dendritic morphogenesis. Our loss-and gain-of-function studies using postnatal electroporation and in utero electroporation indicate that Sox11 is necessary and sufficient for inhibiting dendritic morphogenesis of excitatory neurons in the mouse cerebral cortex during development. Interestingly, we found that precocious suppression of Sox11 expression caused precocious branching of neurites and a neuronal migration defect. We also found that the end of radial migration induced the reduction of Sox11 expression. These findings indicate that suppression of dendritic morphogenesis by Sox11 during radial migration is crucial for the formation of the cerebral cortex.