Mutational spectrum of p53 gene in arsenic-related skin cancers from the blackfoot disease endemic area of Taiwan.
Mutational spectrum of p53 gene in arsenic-related skin cancers from the blackfoot disease endemic area of Taiwan.
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DOI：
10.1038/sj.bjc.6690467
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发表时间：
1999-06
影响因子：
8.8
通讯作者：
Lin, SR
中科院分区：
文献类型：
--
作者：
Hsu, CH;Yang, SA;Wang, JY;Yu, HS;Lin, SR
为了解P53肿瘤抑制基因在台湾黑脚病流行地区砷相关性皮肤癌发生中的作用，我们收集了23例Bowen病患者、7例基底细胞癌(BCC)和9例鳞状细胞癌(SCC)患者的肿瘤标本。结果显示，39%的Bowen病(9/23)、28.6%的基底细胞癌(2/7)和55.6%的鳞癌(5/9)存在P53基因突变。直接测序发现错义突变分别位于密码子149(C→T)、密码子175(G→A)、密码子2 73(G→C)、密码子2 92(T→A)、密码子2 83(T→T)、密码子172(T→C)和密码子2 84(C→A)。此外，在4例中还发现了沉默突变。这些突变分别位于密码子174、253、289和298。免疫组织化学检测发现，43.5%(10/23)的Bowen病、14%(1/7)的基底细胞癌和44%(4/9)的SSC有P53过表达。这些发现显示台湾黑脚病流行地区砷相关皮肤癌的p53基因突变率很高，且突变类型与紫外线诱发的皮肤癌不同。©1999癌症研究活动
To understand the role of p53 tumour suppressor gene in the carcinogenesis of arsenic-related skin cancers from the blackfoot disease endemic area of Taiwan, we collected tumour samples from 23 patients with Bowen's disease, seven patients with basal cell carcinomas (BCC) and nine patients with squamous cell carcinomas (SCC). The result showed that p53 gene mutations were found in 39% of cases with Bowen's disease (9/23), 28.6% of cases with BCC (2/7) and 55.6% of cases with SCC (5/9). Most of the mutation sites were located on exon 5 and exon 8. Moreover, the results from direct sequencing indicated that missense mutations were found at codon 149 (C→T) in one case, codon 175 (G→A) in three cases, codon 273 (G→C) in three cases, codon 292 (T→A) in one case, codon 283 (G→T) in one case, codon 172 (T→C) in one case and codon 284 (C→A) in one case. In addition, silent mutations were also found in four cases. These mutations were located at codons 174, 253, 289 and 298 respectively. In immunohistochemistry analysis, p53 overexpression was found in 43.5% (10/23) of cases with Bowen's disease, 14% (1/7) of cases with BCC and 44% (4/9) of cases with SSC. These findings showed that p53 gene mutation rate in arsenic-related skin cancers from the blackfoot disease endemic area of Taiwan is high and that the mutation types are different from those in UV-induced skin cancers. © 1999 Cancer Research Campaign
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影响因子：
3.8
作者：
BROWN, KG;CHEN, CJ
通讯作者：
CHEN, CJ
DOI：
10.1073/pnas.85.24.9436
发表时间：
1988-12-01
影响因子：
11.1
作者：
INNIS, MA;MYAMBO, KB;BROW, MAD
通讯作者：
BROW, MAD
DOI：
10.1016/0921-8777(93)90054-k
发表时间：
1993-06-01
期刊：
MUTATION RESEARCH
影响因子：
--
作者：
LEECHEN, SF;GURR, JR;JAN, KY
通讯作者：
JAN, KY
DOI：
10.1073/pnas.91.1.360
发表时间：
1994-01-04
影响因子：
11.1
作者：
NAKAZAWA, H;ENGLISH, D;YAMASAKI, H
通讯作者：
YAMASAKI, H
DOI：
10.1073/pnas.93.15.7961
发表时间：
1996-07-23
影响因子：
11.1
作者：
Nataraj, AJ;Black, HS;Ananthaswamy, HN
通讯作者：
Ananthaswamy, HN