The Effects of Surface Roughness of Composite Resin on Biofilm Formation of Streptococcus mutans in the Presence of Saliva
The Effects of Surface Roughness of Composite Resin on Biofilm Formation of Streptococcus mutans in the Presence of Saliva
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DOI：
10.2341/11-371-l
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发表时间：
2012-09-01
影响因子：
2.2
通讯作者：
Ferracane, J. L.
中科院分区：
文献类型：
--
作者：
Park, J. W.;Song, C. W.;Ferracane, J. L.
本研究的目的是研究在唾液存在下，树脂复合材料表面粗糙度对变形链球菌生物膜形成的影响。为了在复合材料上提供均匀的表面粗糙度，通过相对于400粒度碳化硅纸（SR400）、800粒度碳化硅纸（SR800）或载玻片（SRGlass）固化复合材料来制备盘。使用共聚焦激光显微镜检查表面粗糙度。对于生物膜的形成，S.在流体相或表面吸附的唾液存在下，每个盘在含有葡萄糖或蔗糖的生物膜培养基中生长24小时。通过计数细菌的总活菌计数来定量粘附细菌。使用扫描电子显微镜检查生物膜。这项研究表明，SR400比SR800有更深、更大、但更少的凹陷。与SRGlass和SR800相比，SR400上的生物膜形成显著增加。此外，表面粗糙度对生物膜形成量的影响的差异不受唾液或碳水化合物源的存在的显着影响。考虑到在SR400和SR800之间以及SR800和SRGlass之间观察到类似的表面粗糙度差异，本研究表明表面形貌（凹陷的大小和深度）可能在S.变种人
The purpose of this study was to investigate the effects of surface roughness of resin composite on biofilm formation of Streptococcus mutans in the presence of saliva. To provide uniform surface roughness on composites, disks were prepared by curing composite against 400-grit silicon carbide paper (SR400), 800-grit silicon carbide paper (SR800), or a glass slide (SRGlass). The surface roughness was examined using confocal laser microscopy. For biofilm formation, S. mutans was grown for 24 hours with each disk in a biofilm medium with either glucose or sucrose in the presence of fluid-phase or surface-adsorbed saliva. The adherent bacteria were quantified via enumeration of the total viable counts of bacteria. Biofilms were examined using scanning electron microscopy. This study showed that SR400 had deeper and larger, but fewer depressions than SR800. Compared to SRGlass and SR800, biofilm formation was significantly increased on SR400. In addition, the differences in the effect of surface roughness on the amount of biofilm formation were not significantly influenced by either the presence of saliva or the carbohydrate source. Considering that similar differences in surface roughness were observed between SR400 and SR800 and between SR800 and SRGlass, this study suggests that surface topography (size and depth of depressions) may play a more important role than surface roughness in biofilm formation of S. mutans.