Characterization of Sam68-like mammalian proteins SLM-1 and SLM-2: SLM-1 is a Src substrate during mitosis
Characterization of Sam68-like mammalian proteins SLM-1 and SLM-2: SLM-1 is a Src substrate during mitosis
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DOI：
10.1073/pnas.96.6.2710
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发表时间：
1999-03-16
影响因子：
11.1
通讯作者：
Richard, S
中科院分区：
文献类型：
--
作者：
Di Fruscio, M;Chen, TP;Richard, S
Sam 68是bar rC底物下的68-kDa（S），在bar有丝分裂（m）期间相关，是一种具有信号传导特性的RNA结合蛋白，包含GSG（bar RP 33下的（G），bar am 68下的（S），bar LD-1下的（G））结构域。在这里，我们报告了两个（S）下bar am 68-like-（m）下bar ammalian蛋白的克隆。SLM-1和SLM-2这些蛋白质在其GSG结构域中与Sam 68具有约70%的序列同一性。SLM-1和SLM-2具有特征性的Sam 68 SH 2和SH 3结构域结合位点。SLM-1是一种RNA结合蛋白，在有丝分裂期间被Src酪氨酸磷酸化，SLM-1结合p59（fyn）的SH 2和SH 3结构域。Grb-2、磷脂酶C γ-1（PLC γ-1）和/或p120（rasGAP），表明SLM-2可能在有丝分裂期间充当Src的多功能衔接蛋白，SLM-2是不被Src或p59（fyn）酪氨酸磷酸化的RNA结合蛋白。此外，SLM-2不与p59（fyn）、Grb-2 PLC gamma-1或p120（rasGAP）的SH 3结构域结合，这表明SLM-2可能不作为这些蛋白质的衔接蛋白发挥作用。SLM-1和SLM-2的鉴定表明存在Sam 68/SLM家族，其成员具有将信号传导途径与RNA代谢联系起来的潜力。
Sam68, the 68-kDa (S) under bar rC substrate associated during (m) under bar itosis, is an RNA-binding protein with signaling properties that contains a GSG ((G) under bar RP33, (S) under bar am68, (G) under bar LD-1) domain. Here we report the cloning of two (S) under bar am68-like-(m) under bar ammalian proteins. SLM-1 and SLM-2. These proteins have an approximate to 70% sequence identity with Sam68 in their GSG domain. SLM-1 and SLM-2 have the characteristic Sam68 SH2 and SH3 domain binding sites. SLM-1 is an RNA-binding protein that is tyrosine phosphorylated by Src during mitosis, SLM-1 bound the SH2 and SH3 domains of p59(fyn). Grb-2, phospholipase C gamma-1 (PLC gamma-1), and/or p120(rasGAP), suggesting if may function as a multifunctional adapter protein for Src during mitosis, SLM-2 is an RNA-binding protein that is not tyrosine phosphorylated by Src or p59(fyn). Moreover, SLM-2 did not associate with the SH3 domains of p59(fyn), Grb-2 PLC gamma-1, or p120(rasGAP), suggesting that SLM-2 may not function as an adapter protein for these proteins. The identification of SLM-1 and SLM-2 demonstrates the presence of a Sam68/SLM family whose members have the potential to link signaling pathways with RNA metabolism.