Functionality of MDCK kidney tubular cells on flat polymer membranes for biohybrid kidney
Functionality of MDCK kidney tubular cells on flat polymer membranes for biohybrid kidney
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DOI：
10.1023/a:1008994601138
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发表时间：
1998-12
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影响因子：
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通讯作者：
F. Fey-Lamprecht;U. Gross;T. Groth;W. Albrecht;D. Paul;M. Fromm;A. Gitter
中科院分区：
文献类型：
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作者：
F. Fey-Lamprecht;U. Gross;T. Groth;W. Albrecht;D. Paul;M. Fromm;A. Gitter
开发生物杂交人工肾的先决条件是使肾细胞融合生长形成无任何渗漏的上皮单层的基质。传统的细胞培养载体不能适应这一目的，因为它们缺乏足够的机械性能和热稳定性。根据国际标准化组织10993-5标准，用聚砜和聚丙烯腈这两种合适的材料制成了两种无细胞毒性的透过性聚合物膜。将克隆的Madin Darby犬肾细胞系(MDCK)在塑料材料表面和常规细胞培养载体上进行培养。在所有材料中，线粒体和乳酸脱氢酶检测显示MDCK细胞的增殖和活力相似。透射电子显微镜显示肾小管上皮细胞形态正常。完整的屏障功能是在融合的致密上皮中形成细胞间连接的结果，在电子显微镜下可见，并通过测量跨上皮阻力来定量。通过电子显微镜观察细胞生长的一致性，活体阻抗分析显示细胞在整个上皮中生长均匀。结论：聚砜或聚丙烯腈制备的聚合膜是设计生物杂化肾装置的合适基质。(C)1998年克鲁沃学术出版社
The prerequisite for the development of a biohybrid artificial kidney, is a substrate for confluent growth of renal cells forming an epithelial monolayer without any leaks. Conventional cell culture supports cannot be adapted for this purpose, because they lack adequate mechanical properties and thermal stability. From two suitable materials, polysulfone and polyacrylonitrile, two permeable polymeric membranes have been produced that were, according to ISO 10993-5, not cytotoxic. Cloned Madin Darby Canine Kidney (MDCK) cells (an established renal cell line) were cultured on the surface of the plastic materials, and on conventional cell culture supports. With all materials, assays of mitochondrial and lactate dyhydrogenases exhibited similar proliferation and the viability of the MDCK cells. Transmission electron microscopy showed the expression of a normal morphology of kidney tubular cells. Perfect barrier function, consequent on the formation of intercellular junctions in a confluent tight epithelium, was visualized in electron micrographs, and quantified by measurement of the transepithelial resistance. The uniformity of the cells grown was demonstrated in samples by electron microscopy and in the whole epithelium by intravital impedance analysis. It was concluded that polymeric membranes produced from polysulfone or polyacrylonitrile are appropriate substrates in the design of biohybrid kidney devices. © 1998 Kluwer Academic Publishers