Characterization of integrons and resistance genes in multidrug-resistant Salmonella enterica isolated from meat and dairy products in Egypt
Characterization of integrons and resistance genes in multidrug-resistant Salmonella enterica isolated from meat and dairy products in Egypt
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DOI：
10.1016/j.ijfoodmicro.2014.07.031
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发表时间：
2014-10-17
影响因子：
5.4
通讯作者：
Shimamoto, Tadashi
中科院分区：
文献类型：
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作者：
Ahmed, Ashraf M.;Shimamoto, Toshi;Shimamoto, Tadashi
食源性病原体是疾病和死亡的主要原因，特别是在发展中国家。如果细菌获得多重抗药性，问题就会加剧。目前对食源性病原体中抗生素耐药性的程度以及非洲这种耐药性背后的分子机制知之甚少。因此，本研究从分子水平上研究了从埃及不同的街头摊贩、屠宰场、零售市场和屠宰场采集的1600份食品样品(800份肉制品和800份乳制品)中分离的肠炎沙门氏菌的多药耐药机制。在69株分离菌中，47株(68.1%)对至少三类抗菌药表现出多重耐药。多重耐药菌株在肉制品中的发生率(37，69.8%)高于乳制品(10，62.5%)。多重耐药血清型包括：鼠伤寒沙门氏菌24株(34.8%)，肠炎沙门氏菌15株(21.8%)，婴幼儿沙门氏菌7株(10.1%)，非分型沙门氏菌1株(1.4%)。耐药率最高的是氨苄西林(95.7%)，其次是卡那霉素(93.6%)、壮观霉素(93.6%)、链霉素(91.5%)和磺胺甲恶唑/甲氧苄啶(91.5%)。用聚合酶链式反应和DNA测序筛选和鉴定整合子和抗生素耐药基因，1类整合子阳性率为39.1%，2类整合子阳性率为8.7%。75.4%的菌株检测到β-内酰胺酶编码基因，27.5%的菌株检测到质粒介导的喹诺酮类耐药基因。最后，在18.8%的菌株中检测到了氟霉素抗性基因flor。经聚合酶链式反应检测，肉制品和乳制品中均检出鼠伤寒沙门氏菌DT1 04。这是首次报道乳制品中存在许多此类抗性基因的研究。这项研究强调了埃及肉类和乳制品中耐多药肠杆菌的高发病率，并有可能将它们转移到人类身上导致治疗失败。因此，发展中国家应该大幅减少在动物中过度使用抗生素。(C)2014爱思唯尔B.V.保留所有权利。
Foodbome pathogens are a leading cause of illness and death, especially in developing countries. The problem is exacerbated if bacteria attain multidrug resistance. Little is currently known about the extent of antibiotic resistance in foodborne pathogens and the molecular mechanisms underlying this resistance in Africa. Therefore, the current study was carried out to characterize, at the molecular level, the mechanism of multidrug resistance in Salmonella enterica isolated from 1600 food samples (800 meat products and 800 dairy products) collected from different street venders, butchers, retail markets and slaughterhouses in Egypt. Forty-seven out of 69 isolates (68.1%) showed multidrug resistance phenotypes to at least three classes of antimicrobials. The incidence of multidrug-resistant isolates was higher in meat products (37,69.8%) than in dairy products (10, 62.5%). The multidrug-resistant serovars included, S. enterica serovar Typhimurium (24 isolates, 34.8%), S. enterica serovar Enteritidis, (15 isolates, 21.8%), S. enterica serovar Infantis (7 isolates, 10.1%) and S. enterica non-typable serovar (1 isolate, 1.4%). The highest resistance was to ampicillin (95.7%), then to kanamycin (93.6%), spectinomycin (93.6%), streptomycin (91.5%) and sulfamethoxazole/trimethoprim (91.5%). PCR and DNA sequencing were used to screen and characterize integrons and antibiotic resistance genes and 39.1% and 8.7% of isolates were positive for class 1 and class 2 integrons, respectively. beta-lactamase-encoding genes were identified in 75.4% of isolates and plasmid-mediated quinolone resistance genes were identified in 27.5% of isolates. Finally, the florphenicol resistance gene, floR, was identified in 18.8% of isolates. PCR screening identified S. enterica serovar Typhimurium DT1 04 in both meat and dairy products. This is the first study to report many of these resistance genes in dairy products. This study highlights the high incidence of multidrug-resistant S. enterica in meat and dairy products in Egypt, with the possibility of their transfer to humans leading to therapeutic failure. Therefore, the overuse of antibiotics in animals should be drastically reduced in developing countries. (C) 2014 Elsevier B.V. All rights reserved.