FUNCTIONAL DISTINCTIONS BETWEEN YEAST TATA ELEMENTS
FUNCTIONAL DISTINCTIONS BETWEEN YEAST TATA ELEMENTS
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DOI：
10.1128/mcb.9.12.5298
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发表时间：
1989-12-01
影响因子：
5.3
通讯作者：
STRUHL, K
中科院分区：
文献类型：
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作者：
HARBURY, PAB;STRUHL, K
虽然酵母his3启动子区域包含两个TATA元件，tr和tc，但Gcn4和GAL4上游激活蛋白仅通过tr刺激转录。与GAL4结合，含有TATAAA序列的寡核苷酸完全足以发挥tr功能，而该序列的几乎所有单碱基对替换都取消了该元件激活转录的能力。对这些和其他tr元件突变的进一步分析得出了以下结论。首先，TATAAA序列下游的序列对tr功能很重要。其次，双突变体TATTTA可以作为tr元件，即使相应的单突变体TATTAA不能这样做。第三，三个突变具有新的特性，能够与Gcn4一起激活转录，但不与GAL4结合；这一发现表明，Gcn4和GAL4激活可能不是通过相同的机制发生的。通过这些观察，我们解决了是否存在所有基因转录所需的单一TATA结合因子的问题。
Although the yeast his3 promoter region contains two functional TATA elements, TR and TC, the GCN4 and GAL4 upstream activator proteins stimulate transcription only through TR. In combination with GAL4, an oligonucleotide containing the sequence TATAAA is fully sufficient for TR function, whereas almost all single-base-pair substitutions of this sequence abolish the ability of this element to activate transcription. Further analysis of these and other mutations of the TR element led to the following conclusions. First, sequences downstream of the TATAAA sequence are important for the TR function. Second, a double mutant, TATTTA, can serve as a TR element even though the corresponding single mutation, TATTAA, isunable to do so. Third, three mutations have the novel property of being able to activate transcription in combination with GCN4 but not with GAL4; this finding suggests that activation by GCN4 and by GAL4 may not occur by identical mechanisms. From these observations, we address the question of whether there is a single TATA-binding factor required for the transcription of all genes.