ORB IS REQUIRED FOR ANTEROPOSTERIOR AND DORSOVENTRAL PATTERNING DURING DROSOPHILA OOGENESIS
ORB IS REQUIRED FOR ANTEROPOSTERIOR AND DORSOVENTRAL PATTERNING DURING DROSOPHILA OOGENESIS
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DOI：
10.1101/gad.8.5.614
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发表时间：
1994-03-01
影响因子：
10.5
通讯作者：
MCKEARIN, DM
中科院分区：
文献类型：
--
作者：
CHRISTERSON, LB;MCKEARIN, DM
我们描述了突变的orb基因，以前确定为一个大家族的RNA结合蛋白的卵巢特异性成员。强大的orb等位基因逮捕卵子发生之前，卵室形成，卵子发生的早期步骤，而女性突变的母亲效应致命的orb等位基因产卵与腹侧蛋壳结构。在这些突变卵内发育的胚胎显示后部图案缺陷和异常的背腹轴形成。与这些胚胎表型一致，在卵子发生的后期阶段，ORB是卵母细胞内oskar和gurken mRNA不对称分布所必需的。此外，orb和grk或orb和top/DER等位基因的双杂合组合揭示了这些基因中的突变在遗传上相互作用，表明它们参与了一个共同的途径。在野生型雌性的卵母细胞中定位的Orb蛋白在8-10期orb突变卵母细胞中普遍分布。这些数据将在一个模型的背景下进行讨论，该模型提出Orb是将mRNA分子传递到卵母细胞内特定位置的细胞机制的一个组成部分，并且该功能有助于卵子发生过程中的D/V和A/P轴规范。
We describe mutations in the orb gene, identified previously as an ovarian-specific member of a large family of RNA-binding proteins. Strong orb alleles arrest oogenesis prior to egg chamber formation, an early step of oogenesis, whereas females mutant for a maternal-effect lethal orb allele lay eggs with ventralized eggshell structures. Embryos that develop within these mutant eggs display posterior patterning defects and abnormal dorsoventral axis formation. Consistent with such embryonic phenotypes, orb is required for the asymmetric distribution of oskar and gurken mRNAs within the oocyte during the later stages of oogenesis. In addition, double heterozygous combinations of orb and grk or orb and top/DER alleles reveal that mutations in these genes interact genetically, suggesting that they participate in a common pathway. Orb protein, which is localized within the oocyte in wild-type females, is distributed ubiquitously in stage 8-10 orb mutant oocytes. These data will be discussed in the context of a model proposing that Orb is a component of the cellular machinery that delivers mRNA molecules to specific locations within the oocyte and that this function contributes to both D/V and A/P axis specification during oogenesis.