Structure and distribution of nitrite-dependent anaerobic methane oxidation bacteria vary with water tables in Zoige peatlands
Structure and distribution of nitrite-dependent anaerobic methane oxidation bacteria vary with water tables in Zoige peatlands
复制标题
若尔盖泥炭地亚硝酸盐依赖厌氧甲烷氧化细菌的结构和分布随地下水位变化
DOI：
10.1093/femsec/fiaa039
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发表时间：
2020
影响因子：
4.2
通讯作者：
He Zhili
中科院分区：
文献类型：
--
作者：
Zhong Qiuping;Xue Dan;Chen Huai;Liu Liangfeng;He Yixin;Zhu Dan;He Zhili
新近发现的依赖亚硝酸盐的厌氧甲烷氧化(n-达摩)是由NC10门细菌执行的自然生态系统中重要的甲烷汇。然而，全球变化引起的地下水位梯度对泥炭地n-达摩细菌群落的影响还没有得到很好的研究。在此，我们分析了青藏高原若尔盖泥炭地三个不同含水率的地点n-达摩细菌群落的垂直分布(0-100 cm)。利用一个n-达摩细菌特异性16SRRNA基因克隆文库，获得了25个可操作的分类单元，可分为A、B、C、D和E组(以A和B为主)。高WT部位(OTU14和OTU20)和中WT部位(OTU7和OTU8)的优势群为B组，低WT部位(OTU6和OTU5)的优势群为A组。通过高通量测序，我们观察到n-达摩细菌主要分布在地下土壤(50-60和20-30 cm)，其相对丰度在WT较低的地点高于其他两个地点。此外，我们还发现pH和硝酸盐与A组呈正相关，而总有机碳、总氮和氨氮与B组呈正相关。我们的研究为我们理解泥炭地n-达摩细菌对WT梯度的响应提供了新的见解，对全球变化具有重要意义。
The recently discovered nitrite-dependent anaerobic methane oxidation (n-damo) is an important methane sink in natural ecosystems performed by NC10 phylum bacteria. However, the effect of water table (WT) gradient due to global change on n-damo bacterial communities is not well studied in peatlands. Here, we analysed the vertical distribution (0–100 cm) of n-damo bacterial communities at three sites with different WTs of the Zoige peatlands in the Qinghai-Tibetan Plateau. Using an n-damo bacterial specific 16S rRNA gene clone library, we obtained 25 operational taxonomic units (OTUs) that could be divided into Groups A, B, C, D and E (dominated by A and B). The dominant group was Group B at the high (OTU14 and OTU20) and intermediate (OTU7 and OTU8) WT sites and Group A was dominant at the low WT site (OTU6 and OTU5). Using high-throughput sequencing, we observed that n-damo bacteria mainly distributed in subsurface soils (50–60 and 20–30 cm), and their relative abundances were higher at the low WT site than at the other two sites. In addition, we found that pH and nitrate were positively correlated with Group A, while total organic carbon, total nitrogen and ammonia were positively associated with Group B. Our study provides new insights into our understanding of the response of n-damo bacteria to WT gradient in peatlands, with important implications for global change.