Attenuated total reflection Fourier transform infrared studies of redox changes in bovine cytochrome c oxidase: resolution of the redox Fourier transform infrared difference spectrum of heme a(3).
Attenuated total reflection Fourier transform infrared studies of redox changes in bovine cytochrome c oxidase: resolution of the redox Fourier transform infrared difference spectrum of heme a(3).
复制标题
牛细胞色素c氧化酶氧化还原变化的衰减全反射傅里叶变换红外研究：血红素a(3)氧化还原傅里叶变换红外差谱的分辨率。
作者：
P. Rich;J. Breton
用衰减全反射傅里叶变换红外（ATR-FTIR）差谱法对沉积在硅微棱镜表面的牛细胞色素c氧化酶样品进行了研究。该技术在所需的材料量、达到足够的光学稳定性所需的时间以及可以重复添加和去除的反应物范围方面优于透射方法。用ATR-FTIR方法记录了细胞色素c氧化酶未连接态和氰化物连接态的氧化还原差光谱。通过光谱相减，可以将血红素a（3）的氧化还原FTIR差谱与其他金属中心的氧化还原FTIR差谱分辨开来。这种差异的光谱进行比较，与现有的振动和拉曼数据同源氧化酶和血红素A模型化合物。
Attenuated total reflection Fourier transform infrared (ATR-FTIR) difference spectroscopy has been performed on samples of bovine cytochrome c oxidase that have been deposited as a thin film on the surface of a silicon microprism. The technique has several advantages over transmission methods in terms of amount of material required, the time required to reach sufficient optical stability, and the range of reactants that can be repetitively added and removed. The ATR-FTIR method has been used to record redox difference spectra of cytochrome c oxidase in the unligated and cyanide-ligated states. By subtraction of the spectra, the redox FTIR difference spectrum of heme a(3) can be resolved from those of the other metal centers. This difference spectrum is compared with available vibrational and Raman data on homologous oxidases and on heme A model compounds.