ELISA-based measurement of antibody responses and PCR-based detection profiles can distinguish between active infection and early clearance of Borrelia burgdorferi.
ELISA-based measurement of antibody responses and PCR-based detection profiles can distinguish between active infection and early clearance of Borrelia burgdorferi.
复制标题
基于 ELISA 的抗体反应测量和基于 PCR 的检测图谱可以区分伯氏疏螺旋体的活动性感染和早期清除。
DOI：
10.1155/2012/138069
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发表时间：
2012
影响因子：
--
通讯作者：
Wooten,RMark
中科院分区：
文献类型：
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作者：
Lazarus,JohnJ;McCarter,AkishaL;Neifer-Sadhwani,Kari;Wooten,RMark
伯氏疏螺旋体是导致莱姆病的螺旋体细菌。这些研究探讨了目前的研究方法是否使用ELISA来检测B血清转化。组织内的细菌DNA的PCR定量可以准确区分生产性感染与aB。通过先天反应迅速清除的burgdorferi暴露。小鼠即使接受最小剂量的liveB。burgdorferi产生的B.与接受大量热灭活细菌接种的组相比，Burgdorferi特异性IgM和IgG。此外，来自注射不同剂量的killedB.与感染liveB的小鼠相比，burgdorfer识别独特的疏螺旋体抗原。burgdorferi。皮内注射灭活B.结果表明，在接种活菌B的皮肤中，DNA被迅速清除，而在接种活菌B的皮肤中，DNA却始终被检测到。burgdorferi。这些数据表明，基于ELISA的血清学分析和基于PCR的B. Burgdorferiinfection清楚地区分了活细菌的已建立感染和暴露于大量细菌之间，这些细菌通过先天反应迅速清除。
Borrelia burgdorferiis a spirochetal bacterium that causes Lyme disease. These studies address whether current research methods using either ELISA to detect seroconversion toB. burgdorferiantigens or PCR quantification of bacterial DNA within tissues can accurately distinguish between a productive infection versus aB. burgdorferiexposure that is rapidly cleared by the innate responses. Mice receiving even minimal doses of liveB. burgdorferiproduced significantly moreB. burgdorferi‐specific IgM and IgG than groups receiving large inocula of heat‐killed bacteria. Additionally, sera from mice injected with varied doses of killedB. burgdorferirecognized unique borrelial antigens compared to mice infected with liveB. burgdorferi. Intradermal injection of killedB. burgdorferiresulted in rapid DNA clearance from skin, whereas DNA was consistently detected in skin inoculated with viableB. burgdorferi. These data indicate that both ELISA‐based serological analyses and PCR‐based methods of assessingB. burgdorferiinfection clearly distinguish between an established infection with live bacteria and exposure to large numbers of bacteria that are promptly cleared by the innate responses.