Retention of differentiated properties in an established dog kidney epithelial cell line (MDCK).
Retention of differentiated properties in an established dog kidney epithelial cell line (MDCK).
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保留在已建立的狗肾上皮细胞系（MDCK）中的分化特性。
DOI：
10.1083/jcb.81.3.635
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发表时间：
1979-06
期刊：
影响因子：
--
通讯作者：
Saier MH Jr
中科院分区：
文献类型：
--
作者：
Rindler MJ;Chuman LM;Shaffer L;Saier MH Jr
组织培养的MDCK细胞具有远端肾小管上皮细胞的形态特征，形成紧密连接，缺乏多种近端肾小管酶标记物。血管加压素、催产素、前列腺素E_1和E_2、胰升糖素和霍乱毒素可刺激这些细胞的腺苷环化酶。离体膜制剂中激素刺激的腺苷环化酶活性依赖于低浓度的GTP，并具有肾脏酶所需的氯化镁和pH最佳值。这些结果以及环腺苷能促进半囊细胞的形成表明，MDCK细胞系保留了起源于肾上皮细胞的分化特性。将MDCK细胞接种到幼年裸鼠体内，观察到结节持续生长，直至成年。组织学研究显示存在两种细胞类型：正常小鼠成纤维细胞，占固体结节质量的80%-90%；MDCK细胞，形成衬里内部充满液体的腺体的上皮片。电子显微镜分析显示，粘膜细胞表面有面向腺腔的微绒毛，相邻的MDCK细胞由紧密的连接连接，上皮片的浆膜表面有光滑的质膜和连续的基底膜。这些观察结果表明，MDCK细胞在体内保留了其质膜的区域分化和再生肾小管样结构的能力。
Madin-Darby canine kidney (MDCK) cells grown in tissue culture have the morphological properties of distal tubular epithelial cells, form tight junctions, and lack several proximal tubular enzyme markers. Adenylate cyclase in these cells was stimulated by vasopressin, oxytocin, prostaglandins E1 and E2, glucagon, and cholera toxin. Hormone- stimulated adenylate cyclase activity in isolated membrane preparations was dependent on low concentrations of GTP and had the MgCl2 and pH optima expected for the kidney enzyme. The results, as well as the demonstration of enhanced hemicyst formation induced by cyclic AMP, suggest that the MDCK cell line has retained the differentiated properties of the kidney epithelial cell of origin. When MDCK cells were injected into baby nude mice, continuous nodule growth was observed until adulthood was attained. Histological studies revealed the presence of two cell types: normal mouse fibroblasts which comprise 80--90% of the solid nodule mass, and MDCK cells, which formed epithelial sheets lining internal fluid-filled glands. Electron microscope analysis showed that the mucosal surfaces of the cells were characterized by microvilli which faced the lumen of the glands, that adjacent MDCK cells were joined by tight junctions, and that the serosal surfaces of the epithelial sheets were characterized by smooth plasma membranes which were lined by a continuous basement membrane. These observations lead to the conclusion that the MDCK cells retain regional differentiation of their plasma membranes and the ability to regenerate kidney tubule-like structures in vivo.