Three-dimensional particle tracking via bifocal imaging
Three-dimensional particle tracking via bifocal imaging
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DOI：
10.1021/nl0709120
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发表时间：
2007-07-01
期刊：
影响因子：
10.8
通讯作者：
Selvin, Paul R.
中科院分区：
文献类型：
--
作者：
Toprak, Erdal;Balci, Hamza;Selvin, Paul R.
我们引入了一种双焦点成像方法，该方法能够对荧光和非荧光粒子进行三维（3D）追踪。我们通过使用单个电荷耦合器件（CCD）相机同时对一个分子的聚焦平面（用于平面内位置（x，y））和散焦平面（用于平面外位置（z））进行成像来实现这一点。我们将我们的方法应用于多个系统，包括体内黑素体追踪和吞噬的荧光微珠追踪。我们已经以2 - 50毫秒的时间分辨率实现了2 - 5纳米的精度。
We introduce a bifocal imaging method that enables three-dimensional (3D) tracking of both fluorescent and nonfluorescent particles. We accomplish this by simultaneously imaging a focused plane, for in-plane position (x,y), and a defocused plane, for out-of-plane position (z), of a molecule using a single CCD camera. We applied our method to several systems including in vivo melanosome tracking and phagocytosed fluorescent bead tracking. We have achieved 2-5 nm accuracy with a 2-50 ms time resolution.