Replicates in stem cell models-How complicated!
Replicates in stem cell models-How complicated!
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DOI：
10.1002/stem.3237
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发表时间：
2020-06-10
期刊：
影响因子：
5.2
通讯作者：
Teo, Adrian K. K.
中科院分区：
文献类型：
--
作者：
Chan, Jun-Wei;Teo, Adrian K. K.
当前基于人类多能干细胞（hPSC）的研究的复杂性。hPSC研究开始招募携带疾病相关基因变体的患者，所述疾病相关基因变体可增加对疾病发展的易感性。然后进行体细胞重编程以衍生患者特异性人诱导多能干细胞（hiPSC），然后逐步定向分化为相关细胞类型，然后进行定性/定量测定以评估健康和患病hiPSC之间的表型或基因表达差异。
Current complexities in human pluripotent stem cell (hPSC)-based studies. hPSC studies begin with the recruitment of patients harboring disease-associated gene variant(s) that may increase susceptibility to disease development. Somatic reprogramming is then performed to derive patient-specific human induced pluripotent stem cells (hiPSCs), followed by step-wise directed differentiation to a relevant cell type before qualitative/quantitative assays are performed to assess for phenotypic or gene expression differences between the healthy and diseased hiPSCs.