Differences in the transcriptional profiles of human cumulus cells isolated from MI and MII oocytes of patients with polycystic ovary syndrome
Differences in the transcriptional profiles of human cumulus cells isolated from MI and MII oocytes of patients with polycystic ovary syndrome
复制标题
从多囊卵巢综合征患者的 MI 和 MII 卵母细胞中分离出的人卵丘细胞转录谱的差异。
DOI：
10.1530/rep-13-0005
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发表时间：
2013-06-01
期刊：
影响因子：
3.8
通讯作者：
Chen, Lili
中科院分区：
文献类型：
--
作者：
Huang, Xin;Hao, Cuifang;Chen, Lili
多囊卵巢综合征(PCOS)是女性常见的内分泌代谢紊乱。内分泌和卵巢内旁分泌相互作用的异常可能改变卵泡发生过程中卵母细胞发育的微环境，降低无排卵性不孕症和妊娠丢失患者卵母细胞的发育能力。在这种微环境中，卵母细胞与周围的卵丘细胞(CCS)之间的串扰对于获得卵母细胞的能力至关重要。本研究的目的是利用人类基因组U133 Plus 2.0芯片研究PCOS患者体外受精周期中CCS的基因表达谱。共有59个基因在两个CC组中差异表达。这些基因中的大多数被确定参与以下一个或多个途径：受体相互作用、钙信号、代谢和生物合成、焦点黏附、黑素生成、白细胞跨内皮细胞迁移、Wnt信号和2型糖尿病。根据芯片中不同的表达水平及其可能的功能，筛选出6个差异表达基因(LHCGR、ANGPTL1、tnik、GRIN2A、sFRP4和SOCS3)，并进行定量RT-PCR分析。定量逆转录聚合酶链式反应的结果与芯片数据一致。此外，分子标记(LHCGR、TNIK和SOCS3)与从胚胎到囊胚期的发育潜能相关，并被认为是PCOS患者胚胎存活的生物标志物。我们的结果可能在临床上具有重要意义，因为它们为PCOS患者选择合格的卵母细胞/胚胎提供了一种新的潜在策略。
Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in women. The abnormalities of endocrine and intra-ovarian paracrine interactions may change the microenvironment for oocyte development during the folliculogenesis process and reduce the developmental competence of oocytes in PCOS patients who are suffering from anovulatory infertility and pregnancy loss. In this microenvironment, the cross talk between an oocyte and the surrounding cumulus cells (CCs) is critical for achieving oocyte competence. The aim of our study was to investigate the gene expression profiles of CCs obtained from PCOS patients undergoing IVF cycles in terms of oocyte maturation by using human Genome U133 Plus 2.0 microarrays. A total of 59 genes were differentially expressed in two CC groups. Most of these genes were identified to be involved in one or more of the following pathways: receptor interactions, calcium signaling, metabolism and biosynthesis, focal adhesion, melanogenesis, leukocyte transendothelial migration, Wnt signaling, and type 2 diabetes mellitus. According to the different expression levels in the microarrays and their putative functions, six differentially expressed genes (LHCGR, ANGPTL1, TNIK, GRIN2A, SFRP4, and SOCS3) were selected and analyzed by quantitative RT-PCR (qRT-PCR). The qRT-PCR results were consistent with the microarray data. Moreover, the molecular signatures (LHCGR, TNIK, and SOCS3) were associated with developmental potential from embryo to blastocyst stage and were proposed as biomarkers of embryo viability in PCOS patients. Our results may be clinically important as they offer a new potential strategy for competent oocyte/embryo selection in PCOS patients.