EIN3-dependent regulation of plant ethylene hormone signaling by two Arabidopsis F box proteins:: EBF1 and EBF2
EIN3-dependent regulation of plant ethylene hormone signaling by two Arabidopsis F box proteins:: EBF1 and EBF2
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DOI：
10.1016/s0092-8674(03)00968-1
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发表时间：
2003-12-12
期刊：
影响因子：
64.5
通讯作者：
Genschik, P
中科院分区：
文献类型：
--
作者：
Potuschak, T;Lechner, E;Genschik, P
植物激素乙烯调节广泛的发育过程以及植物对胁迫和病原体的反应。 Genetic studies in Arabidopsis led to a partial elucidation of the mechanisms of ethylene action.乙烯信号转导起始于乙烯与乙烯受体家族的结合，终止于涉及植物特异性转录因子 EIN3/EIL 和 ERF 家族的转录级联。在这里，我们鉴定了两种拟南芥 F 盒蛋白，称为 EBF1 和 EBF2，它们与 EIN3/EIL 转录因子发生物理相互作用。 EBF1 过度表达导致植物对乙烯不敏感。相比之下，携带 ebf1 和 ebf2 突变的植物表现出组成型乙烯反应，并在缺乏激素的情况下积累 EIN3 蛋白。我们的工作将 EBF1 和 EBF2 置于乙烯反应途径的遗传框架内，并支持乙烯作用依赖于 EIN3 蛋白稳定的模型。
The plant hormone ethylene regulates a wide range of developmental processes and the response of plants to stress and pathogens. Genetic studies in Arabidopsis led to a partial elucidation of the mechanisms of ethylene action. Ethylene signal transduction initiates with ethylene binding at a family of ethylene receptors and terminates in a transcription cascade involving the EIN3/EIL and ERF families of plant-specific transcription factors. Here, we identify two Arabidopsis F box proteins called EBF1 and EBF2 that interact physically with EIN3/EIL transcription factors. EBF1 overexpression results in plants insensitive to ethylene. In contrast, plants carrying the ebf1 and ebf2 mutations display a constitutive ethylene response and accumulate the EIN3 protein in the absence of the hormone. Our work places EBF1 and EBF2 within the genetic framework of the ethylene-response pathway and supports a model in which ethylene action depends on EIN3 protein stabilization.