Transformation of malaria parasites by the spontaneous uptake and expression of DNA from human erythrocytes
Transformation of malaria parasites by the spontaneous uptake and expression of DNA from human erythrocytes
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DOI：
10.1093/nar/29.3.850
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发表时间：
2001-02-01
影响因子：
14.9
通讯作者：
Wellems, TE
中科院分区：
文献类型：
--
作者：
Deitsch, KW;Driskill, CL;Wellems, TE
细胞外DNA的摄取和表达已被确立为细菌物种之间基因水平转移的机制。这种转移可以支持有利元素的获取，包括影响感染性生物体与其宿主之间相互作用的决定因素。在这里，我们表明，红细胞阶段恶性疟原虫疟疾寄生虫自发地采取从宿主细胞质的DNA进入其细胞核。我们已经利用这一发现在恶性疟原虫中产生报告基因表达水平，其与通过目前用于杀灭疟疾寄生虫的电穿孔方案所获得的水平相比有实质性改善。当将寄生虫置于含有编码人二氢叶酸还原酶序列的质粒的红细胞的细胞培养物中时，寄生虫被转化为抗药性状态。本文报道的研究结果表明，疟疾基因组可能不断暴露于宿主红细胞中残留核物质的外源DNA。
The uptake and expression of extracellular DNA has been established as a mechanism for horizontal transfer of genes between bacterial species. Such transfer can support acquisition of advantageous elements, including determinants that affect the interactions between infectious organisms and their hosts. Here we show that erythrocyte-stage Plasmodium falciparum malaria parasites spontaneously take up DNA from the host cell cytoplasm into their nuclei. We have exploited this finding to produce levels of reporter expression in P.falciparum that are substantially improved over those obtained by electroporation protocols currently used to transfect malaria parasites, Parasites were transformed to a drug-resistant state when placed into cell culture with erythrocytes containing a plasmid encoding the human dihydrofolate reductase sequence. The findings reported here suggest that the malaria genome may be continually exposed to exogenous DNA from residual nuclear material in host erythrocytes.