Comparative transcriptome analysis between heat-tolerant and sensitive Pyropia haitanensis strains in response to high temperature stress
Comparative transcriptome analysis between heat-tolerant and sensitive Pyropia haitanensis strains in response to high temperature stress
复制标题
DOI：
10.1016/j.algal.2017.11.026
复制
发表时间：
2018-01
期刊：
影响因子：
--
通讯作者：
Wenlei Wang;Yinghui Lin;Fei Teng;De-hua Ji;Yan Xu;Chang-sheng Chen;Chaotian Xie
中科院分区：
文献类型：
--
作者：
Wenlei Wang;Yinghui Lin;Fei Teng;De-hua Ji;Yan Xu;Chang-sheng Chen;Chaotian Xie
Pyropia haitanensisfarms in South China often suffer from sustained high temperatures in autumn, which is the early seeding period, resulting in disease, premature senility, and eventual decay, ultimately leading to a substantial reduction in yield. However, the molecular mechanisms underlying the response to high temperature stress inP. haitanensisremain unknown. In the present study, we used the Illumina sequencing platform to examine the transcriptional profiles of heat-tolerant (THT) and heat-sensitive (WHT)P. haitanensisstrains exposed to different durations of high temperature stress. THT exhibited more differentially expressed genes and greater fold-changes in transcripts under sustained temperature stress, suggesting that this strain is better able to increase transcriptional regulation in response to high temperature stress than WHT. Furthermore, heat stress induced different expression patterns in the thalli of the two strains. THT was able to maintain or increase the activities of energy metabolism, antioxidant systems, and phosphatidylinositol signal transduction to resist heat stress, while these processes were dramatically reduced in WHT, resulting in the thalli being unable to survive under sustained high temperatures. The present data provide a better understanding of the mechanisms underlying the response ofP. haitanensisto high temperature stress, which may facilitate the development of technologies and breeding strategies for improving thermotolerance inPyropia.
