Decoding Human Megakaryocyte Development
Decoding Human Megakaryocyte Development
复制标题
解码人类巨核细胞的发育
DOI：
10.1016/j.stem.2020.11.006
复制
发表时间：
2021-03-04
期刊：
影响因子：
23.9
通讯作者：
Zhou, Jiaxi
中科院分区：
文献类型：
--
作者：
Wang, Hongtao;He, Jian;Zhou, Jiaxi
尽管我们越来越了解胚胎免疫发育，罕见的早期巨核细胞（MK）仍然相对不足。在这里，我们使用单细胞RNA测序的人MK从胚胎卵黄囊（YS）和胎肝（FL）的转录组，细胞异质性和早期巨核细胞的发育轨迹的特点。在YS和FL，我们发现异质MK亚群不同的发展路线和基因表达模式，可以反映早期的功能专业化。有趣的是，我们在体内鉴定了一个CD42b（+）CD14（+）MK亚群，其表现出与免疫应答相关的基因的高表达，并且也可以在体外来源于人胚胎干细胞（hESC）。此外，我们确定THBS1作为MK偏向胚胎内皮细胞的早期标志物。总之，我们提供了重要的见解和宝贵的资源解剖的分子和细胞程序的基础早期人类巨核细胞。
Despite our growing understanding of embryonic immune development, rare early megakaryocytes (MKs) remain relatively understudied. Here we used single-cell RNA sequencing of human MKs from embryonic yolk sac (YS) and fetal liver (FL) to characterize the transcriptome, cellular heterogeneity, and developmental trajectories of early megakaryopoiesis. In the YS and FL, we found heterogeneous MK subpopulations with distinct developmental routes and patterns of gene expression that could reflect early functional specialization. Intriguingly, we identified a subpopulation of CD42b(+)CD14(+) MKs in vivo that exhibit high expression of genes associated with immune responses and can also be derived from human embryonic stem cells (hESCs) in vitro. Furthermore, we identified THBS1 as an early marker for MK-biased embryonic endothelial cells. Overall, we provide important insights and invaluable resources for dissection of the molecular and cellular programs underlying early human megakaryopoiesis.