Towards a Quantitative Understanding of Protein–Lipid Bilayer Interactions at the Single Molecule Level: Opportunities and Challenges
Towards a Quantitative Understanding of Protein–Lipid Bilayer Interactions at the Single Molecule Level: Opportunities and Challenges
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DOI：
10.1007/s00232-020-00151-0
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发表时间：
2020-11
期刊：
影响因子：
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通讯作者：
G. King;I. Kosztin
中科院分区：
文献类型：
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作者：
G. King;I. Kosztin
蛋白质-脂质界面是生物学中最基本的界面之一。然而，应用传统技术来研究这些系统的生物物理属性是具有挑战性的，并留下了许多未知数。例如，当一条多肽链靠近一个液体脂质双层时，它所经历的动力学途径和能量景观是什么？在这里，我们回顾了实验和理论的进展，我们已经在解决这个问题，从单分子的角度来看。还讨论了一些剩余的障碍。
AbstractProtein–lipid interfaces are among the most fundamental in biology. Yet applying conventional techniques to study the biophysical attributes of these systems is challenging and has left many unknowns. For example, what is the kinetic pathway and energy landscape experienced by a polypeptide chain when in close proximity to a fluid lipid bilayer? Here we review the experimental and theoretical progress we have made in addressing this question from a single molecule perspective. Some remaining impediments are also discussed.Graphic Abstract