Salt Extraction of Soluble HL-A Antigens
Salt Extraction of Soluble HL-A Antigens
复制标题
可溶性 HL-A 抗原的盐提取
作者：
R. A. Reisfeld;M. A. Pellegrino;B. Kahan
Extraction of cultured human lymphoid cells with hypertonic salt solutions (3 molar potassium chloride) resulted in high recoveries of membrane-associated histocompatibility (HL-A) antigens in soluble form with potent activity and marked immunologic specificity. The active principle was purified by preparative acrylamide-gel electrophoresis. Application of the hypertonic salt extraction method is now yielding sufficient HL-A antigen to begin the elucidation of the molecular basis of transplantation individuality.
