Identification and functional characterization of BTas transactivator as a DNA-binding protein.
Identification and functional characterization of BTas transactivator as a DNA-binding protein.
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DOI：
10.1016/j.virol.2010.05.037
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发表时间：
2010-09
期刊：
影响因子：
3.7
通讯作者：
Juan Tan;Peng Hao;R. Jia;Wei Yang;R. Liu;Jinzhong Wang;Zhen Xi;Y. Geng;W. Qiao
中科院分区：
文献类型：
--
作者：
Juan Tan;Peng Hao;R. Jia;Wei Yang;R. Liu;Jinzhong Wang;Zhen Xi;Y. Geng;W. Qiao
牛泡沫病毒（bovine foamy virus，BFV）基因组编码一种转录反式激活因子，即BTas，它通过与病毒长末端重复序列启动子（long-terminal repeat promoter，LTR）和内部启动子（internal promoter，IP）结合而显著增强基因表达。在这份报告中，我们的特点是BFV BTas的功能结构域。BTas包含两个主要的功能结构域：N-末端DNA结合结构域（残基1-133）和C-末端激活结构域（残基198-249）。完整的BTas反应区域被映射到LTR的-380/-140和IP的9205/9276位置。在BFV LTR的−368/−346、−327/−307、−306/−285和−186/−165位置鉴定了四个BTas响应元件，在BFV IP的9243/9264位置鉴定了一个元件。与其他泡沫病毒不同，BFV中的5个BTas反应元件具有明显的序列同源性。这些数据表明，在复杂的逆转录病毒，BFV似乎有一个独特的反式激活机制。
The genome of bovine foamy virus (BFV) encodes a transcriptional transactivator, namely BTas, that remarkably enhances gene expression by binding to the viral long-terminal repeat promoter (LTR) and internal promoter (IP). In this report, we characterized the functional domains of BFV BTas. BTas contains two major functional domains: the N-terminal DNA-binding domain (residues 1–133) and the C-terminal activation domain (residues 198–249). The complete BTas responsive regions were mapped to the positions −380/−140 of LTR and 9205/9276 of IP. Four BTas responsive elements were identified at the positions −368/−346, −327/−307, −306/−285 and −186/−165 of the BFV LTR, and one element was identified at the position 9243/9264 of the BFV IP. Unlike other foamy viruses, the five BTas responsive elements in BFV shared obvious sequence homology. These data suggest that among the complex retroviruses, BFV appears to have a unique transactivation mechanism.