Gestational changes in calcitonin gene-related peptide, nerve growth factor, and its receptors in rat dorsal root ganglia.
Gestational changes in calcitonin gene-related peptide, nerve growth factor, and its receptors in rat dorsal root ganglia.
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妊娠期大鼠背根神经节降钙素基因相关肽、神经生长因子及其受体的变化。
DOI：
10.1095/biolreprod65.5.1601
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发表时间：
2001
影响因子：
3.6
通讯作者：
Yallampalli,C
中科院分区：
文献类型：
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作者：
Lanlua,P;Gangula,PR;Taglialatela,G;Yallampalli,C
在背根节(DRG)细胞培养中，卵巢激素和神经生长因子(NGF)的存在会增加降钙素基因相关肽(CGRP)的水平。此外，卵巢切除大鼠注射卵巢激素后，DRG中两种NGF受体TrkA和p75NTR的水平增加。因此，我们推测，怀孕期间卵巢激素水平的增加可能会增加DRG中CGRP和NGF受体的合成。用放射免疫法、Western印迹法和NGF免疫法分别测定孕期、产后2d和去卵巢后大鼠背根神经节中CGRP、TrkA、p75NTR和NGF的水平。孕期大鼠背根神经节CGRP水平显著高于产后第2天或去卵巢大鼠。TrkA和p75NTRin DRG的水平在妊娠期间均升高，并在产后第2天保持较高水平，但CGRP水平下降。NGF水平在妊娠第18天达到统计学意义上的峰值，在产后第2天没有显著下降。妊娠期卵巢类固醇激素水平升高可能与CGRP的合成有关，而产后CGRP合成的减少似乎与NGF及其受体无关。
In dorsal root ganglia (DRG) cell cultures, levels of calcitonin gene-related peptide (CGRP) are increased in the presence of ovarian hormones and nerve growth factor (NGF). In addition, injection of ovariectomized rats with ovarian hormones led to an increase in levels of two NGF receptors, TrkA and p75NTR, in DRG. Thus, we hypothesized that increased levels of ovarian hormones during pregnancy may elevate the synthesis of CGRP and NGF receptors in the DRG. DRG harvested from rats on specific days of pregnancy, on Day 2 postpartum, and after ovariectomy were subjected to radioimmunoassay, Western blot analysis, and NGF immunoassay to determine levels of CGRP, TrkA and p75NTR, and NGF, respectively. CGRP levels in rat DRG were significantly higher during pregnancy than at Day 2 postpartum or in ovariectomized rats. Levels of both TrkA and p75NTRin DRG increased during pregnancy and remained elevated at Day 2 postpartum, but CGRP levels declined. Levels of NGF reached a statistically significant peak at Day 18 of gestation, and were not significantly reduced at Day 2 postpartum. Increased levels of ovarian steroid hormones during pregnancy may be involved in the synthesis of CGRP, however, the postpartum decreases in CGRP synthesis appear to be unrelated to NGF and its receptors.