Lentiviral vectors: optimization of packaging, transduction and gene expression
Lentiviral vectors: optimization of packaging, transduction and gene expression
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DOI：
10.1002/jgm.501
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发表时间：
2004-02-01
影响因子：
3.5
通讯作者：
Delenda, C
中科院分区：
文献类型：
--
作者：
Delenda, C
基于逆转录病毒（包括致癌逆转录病毒和慢病毒）的基因转移载体为外源基因在哺乳动物细胞中的递送、整合和表达提供了有效手段。慢病毒（LV）载体在非分裂细胞的情况下提供有吸引力的基因递送载体。本文综述了不同的优化LV遗传系统，已开发的日期。在所有情况下，LV衍生载体的生产由遗传分裂基因表达设计组成。特别需要的病毒元件是（i）至少由gag-pot基因组成的LV包装辅助蛋白，（ii）含有转基因表达盒的LV转移载体RNA，和（iii）异源糖蛋白。虽然前两种病毒元件的遗传要求和性能将在本文中讨论，但后一种元件相对于LV载体的包膜假型化将不作进一步描述（参见图1）。在这个问题上，由Cosset评论）。版权所有（C）2004约翰威利父子有限公司。
Gene transfer vectors based on retroviruses including oncogenic retroviruses and lentiviruses provide effective means for the delivery, integration and expression of exogenous genes in mammalian cells. Lentiviral (LV) vectors provide attractive gene delivery vehicles in the context of non-dividing cells. This review summarizes the different optimized LV genetic systems that have been developed to date. in all cases, the production of LV-derived vectors consists of a genetically split gene expression design. The viral elements that are specifically required are (i) the LV packaging helper proteins consisting of at least the gag-pot genes, (ii) the LV transfer vector RNA containing the transgene expression cassette, and (iii) an heterologous glycoprotein. While the genetic requirements and performances of the two former viral elements will be treated herein, the latter element relative to the envelope pseudotyping of LV vectors will not be further described (cf. review by Cosset in this issue). Copyright (C) 2004 John Wiley Sons, Ltd.