Isolation of Cerebral Endothelial Cells from CCM1/KRIT1 Null Mouse Brain.
Isolation of Cerebral Endothelial Cells from CCM1/KRIT1 Null Mouse Brain.
复制标题
从 CCM1/KRIT1 空鼠脑中分离脑内皮细胞。
DOI：
10.1007/978-1-0716-0640-7_19
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Glading,AngelaJ
中科院分区：
文献类型：
--
作者：
Nobiletti,Nicholas;Glading,AngelaJ
脑海绵状血管畸形（CCM）是由脑微血管内皮细胞群的变化驱动的。CCM的小鼠模型已经成功地在体内重现了疾病;然而，由于活体动物中涉及的组织有限，因此在体内解剖疾病发病机制和分子机制具有挑战性。因此，需要体外组织培养模型。该方案旨在促进从整个小鼠脑组织中分离脑微血管内皮细胞。该方案利用木瓜蛋白酶进行较短的单一消化步骤，以最大限度地提高细胞回收率和活力。使用这种技术，我们能够从小鼠CCM模型中分离出细胞，在该模型中，CCM蛋白的缺失是由出生时Cre介导的重组驱动的，并导致成年动物中的CCM样血管畸形。
Cerebral cavernous malformation (CCM) is driven by changes in the cerebral microvascular endothelial cell population. Mouse models of CCM have successfully recapitulated the disease in vivo; however, dissection of the disease pathogenesis and molecular mechanism is challenging in vivo due to limited access to the involved tissue in live animals. Therefore, in vitro tissue culture models are required. This protocol is designed to facilitate the isolation of cerebral microvascular endothelial cells from whole murine brain tissue. The protocol utilizes papain for a shorter, single digestion step to maximize cell recovery and viability. Using this technique, we are able to isolate cells from a murine CCM model in which the absence of CCM proteins is driven by Cre-mediated recombination at birth, and results in CCM-like vascular malformations in adult animals.
影响因子：
15.9
作者：
Chan, Aubrey C.;Drakos, Stavros G.;Li, Dean Y.
通讯作者：
Li, Dean Y.