Reference values of biochemical and hematological parameters for Guizhou minipigs
Reference values of biochemical and hematological parameters for Guizhou minipigs
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DOI：
10.1258/ebm.2011.010283
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发表时间：
2011-04-01
影响因子：
3.2
通讯作者：
Lu, Yanrong
中科院分区：
文献类型：
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作者：
Chen, Younan;Qin, Shengfang;Lu, Yanrong
猪不仅是重要的经济畜牧动物，也是生物医学研究和异种移植的有价值的模型动物。使用猪模型时，需要临床生化和血液学参数的参考值才能准确解释数据。本研究采集了 54 头健康的中国贵州小型猪的全血样本。我们分析了常规生化和血液学参数以及特殊凝血参数，包括血栓弹力图和凝血因子活性，并提出了这些参数的基线值。这些数据为在生物医学研究中使用小型猪作为动物模型的研究人员提供了有价值的信息，并为兽医和牲畜生产者提供了有用的生理数据。我们还将小型猪的所有结果与健康人类的相应数据进行了比较。小型猪的胆红素、尿酸和胆固醇水平显着低于人类（分别为人类水平的14%、0.086%和48%），而血清酶水平则远高于人类（例如，小型猪的羟丁酸脱氢酶和肌酸激酶水平分别比人类参考值高19倍和8.4倍）。小型猪的红细胞计数、血小板计数和白细胞计数显着高于人类。小型猪的凝血因子 VII 和凝血因子 X 的凝血活性高于人类。在小型猪和人类之间观察到的许多参数的显着差异表明器官和组织方面存在巨大的种间差异。这些差异值得在涉及小型猪的生物医学研究中得到更多关注，特别是在猪到人的移植领域。
The pig is not only an economically important livestock animal but is also a valuable model animal for biomedical research and xenotransplantation. Reference values for clinical biochemical and hematological parameters are required for accurate data interpretation while using a pig model. In this study, whole blood samples were collected from 54 healthy Chinese Guizhou minipigs. We analyzed routine biochemical and hematological parameters and special coagulation parameters, including thrombelastography and coagulation factor activities, and have presented the baseline values of these parameters. These data provide valuable information for investigators using minipigs as animal models in biomedical studies and useful physiological data for veterinarians and livestock producers. We also compared all the results for the minipigs with the corresponding data from healthy humans. The bilirubin, uric acid and cholesterol levels of minipigs were significantly lower than those of humans (14%, 0.086% and 48% of human levels, respectively), whereas the serum enzyme levels were much higher than those in humans (e.g. the hydroxybutyrate dehydrogenase and creatine kinase levels of the minipigs were 19- and 8.4-fold higher than the human reference values). The red blood cell counts, platelet counts and white blood cell counts of the minipigs were significantly higher than those of the humans. The coagulation activities of factor VII and factor X were higher in minipigs than in humans. The significant differences observed between minipigs and humans for many of these parameters suggest substantial interspecies disparities in organs and tissues. These differences merit greater attention in biomedical research involving minipigs, particularly in the area of pig-to-human transplantation.