Improved Cellular Immune Response Elicited by a Ubiquitin-Fused DNA Vaccine Against Mycobacterium tuberculosis
Improved Cellular Immune Response Elicited by a Ubiquitin-Fused DNA Vaccine Against Mycobacterium tuberculosis
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泛素融合 DNA 疫苗可改善结核分枝杆菌细胞免疫反应
DOI：
10.1089/dna.2011.1309
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发表时间：
2012-04-01
影响因子：
3.1
通讯作者：
Liu, Qiuhong
中科院分区：
文献类型：
--
作者：
Wang, Qingmin;Lei, Chengxiang;Liu, Qiuhong
本研究评价了泛素（Ub）融合的MPT 64 DNA疫苗诱导的抗结核分枝杆菌的免疫应答。分别用编码MPT 64蛋白的质粒DNA、UbGR-MPT 64 DNA疫苗（UbGR-MPT 64）和阴性DNA疫苗免疫BALB/c小鼠。MPT 64 DNA疫苗免疫诱导Th 1极化的免疫应答。与未融合DNA疫苗相比，UbGR-MPT 64融合DNA疫苗免疫小鼠后，Th 1型细胞因子（IFN-γ）的产生和增殖性T细胞反应显著增强。此外，该融合DNA疫苗还导致IgG 2a与IgG 1的相对比例增加和T细胞的细胞毒性。脾细胞的IFN-g细胞内染色表明，UbGR-mpt 64融合DNA疫苗活化了CD 4+和CD 8 + T细胞，特别是CD 8 + T细胞。因此，本研究表明，UbGR-MPT 64融合DNA疫苗接种能提高抗原特异性细胞免疫应答，有助于预防结核病的发生。
This study evaluated the immune response elicited by a ubiquitin (Ub)-fused MPT64 DNA vaccine against Mycobacterium tuberculosis. BALB/c mice were vaccinated with plasmid DNA encoding MPT64 protein, Ubfused MPT64 DNA vaccine (UbGR-MPT64), and negative DNA vaccines, respectively. MPT64 DNA vaccine immunization induced a Thl-polarized immune response. The production of Thl-type cytokine (interferon-gamma [ IFN-gamma]) and proliferative T cell responses were enhanced significantly in mice immunized with UbGR-MPT64 fusion DNA vaccine, compared with nonfusion DNA vaccine. Moreover, this fusion DNA vaccine also resulted in an increased relative ratio of IgG2a to IgGl and the cytotoxicity of T cells. IFN-g intracellular staining of splenocytes indicated that UbGR-mpt64 fusion DNA vaccine activated CD4+ and CD8+ T cells, particularly CD8+ T cells. Thus, this study demonstrated that the UbGR-MPT64 fusion DNA vaccine inoculation could improve antigen-specific cellular immune responses, which is helpful for protection against TB.