STRUCTURE OF CENTRAL REGION IN SYNAPTONEMAL COMPLEXES OF HAMSTER AND CRICKET SPERMATOCYTES
STRUCTURE OF CENTRAL REGION IN SYNAPTONEMAL COMPLEXES OF HAMSTER AND CRICKET SPERMATOCYTES
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DOI：
10.1083/jcb.56.1.145
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发表时间：
1973-01-01
影响因子：
7.8
通讯作者：
MOSES, MJ
中科院分区：
文献类型：
--
作者：
SOLARI, AJ;MOSES, MJ
采用全表面涂布法结合阴性染色法对金仓鼠和蟋蟀精母细胞二价体的精细结构进行了研究。突触复合体的元素显示了在其他制备过程中所缺乏的结构细节。在两个物种的突触复合体的中心区域发现的横向细丝是直的，宽度相似，为1 6 - 8纳米，这些细丝主要以束状出现。在两个物种中，中心成分的结构不同。在仓鼠的二价体中，它是由1.6-1 8纳米宽的纵向细丝和少量类似于蟋蟀的横向成分的无定形物质组成的。中心元件包含与中心区域横向细丝连续的横向原纤维，以及主要沿中心元件两侧分布的无定形物质。突触复合物中心区域的所有成分都对胰腺dna酶具有抗性。横向丝的广泛存在和密切的种间形态和组织化学相似性表明，它们起着重要的作用，可能与通过横向丝将突触二价体结合在一起有关。本文还讨论了目前的突触复合体模型所受到的限制。
The fine structure of bivalents from golden hamster and house cricket spermatocytes has been studied with a whole mount surface-spreading method combined with negative staining. The elements of the synaptonemal complex show detail of structure which is absent in other preparative procedures. The transverse filaments found in the central region of the synaptonemal complex from both species are straight and have a similar width, 1 6–1 8 nm These filaments occur mainly in bundles The central element differs in architecture in the two species In hamster bivalents it is formed of longitudinal stretches of filaments 1.6–1 8 nm wide and a small amount of an amorphous material similar to that of the lateral elements In the cricket, the central element contains transverse fibrils which are continuous with the transverse filaments of the central region, and an amorphous material lying mainly along the sides of the central element All of the components of the central region of the synaptonemal complex are resistant to pancreatic DNase. The overlapping ends of the transverse filaments, together with additional protein material, make up the central element The widespread occurrence and close morphological and histochemical interspecies similarities of the transverse filaments indicate that they serve an essential role, probably one concerned with holding synapsed bivalents together via the lateral elements. Restrictions placed by the observations reported here on current models of the synaptonemal complex are discussed.