MORPHOLOGICAL CHARACTERIZATION OF TENDON DEVELOPMENT DURING CHICK EMBRYOGENESIS - MEASUREMENT OF BIREFRINGENCE RETARDATION
MORPHOLOGICAL CHARACTERIZATION OF TENDON DEVELOPMENT DURING CHICK EMBRYOGENESIS - MEASUREMENT OF BIREFRINGENCE RETARDATION
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DOI：
10.1016/0141-8130(85)90034-0
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发表时间：
1985-01-01
影响因子：
8.2
通讯作者：
SILVER, FH
中科院分区：
文献类型：
--
作者：
MCBRIDE, DJ;HAHN, RA;SILVER, FH
通过形态学观察以及双折射延迟、平均纤维轮廓宽度和细胞体积分数的物理测量，对鸡后肢伸肌腱的发育进行了表征。在胚胎期第7、10、14和17天以及孵化后1 - 1.5天进行了观察。微观解剖观察表明，肌腱微观解剖结构是依次发育的：在第7天胚胎中存在由单轴细胞框架和不连续的胶原纤维束组成的结构；到胚胎第10天，出现了连续的双折射胶原纤维网络，以及肌腱束形成和卷曲发育的早期迹象；在胚胎发育第14天观察到肌腱基本细胞结构完成。肌腱中的胶原沉积首先涉及肌腱成纤维细胞的纵向组织，然后是横向组织。与肌腱的渐进性解剖发育相关的是双折射延迟增加、平均胶原纤维轮廓宽度增加以及细胞体积分数降低。单位厚度的双折射延迟没有变化。尽管观察到纤维尺寸增大，但纤维的原纤维堆积密度保持不变。显然，通过使用偏振光测量双折射延迟，可以对体内的胶原原纤维生成进行定量研究。
Morphological observations and physical measurements of birefringence retardation, mean fiber profile width and cell volume fraction were used to characterize chick hind limb extensor tendon development. Observations were made at days 7, 10, 14 and 17 embryologic and 1-1.5 post-hatching. Microanatomical observations illustrated a sequential development of tendon microanatomy consisting of a uniaxial cellular framework with discontinuous collagen fibril bundles present in day 7 embryos; a continuous network of birefringent collagen fibers, and early evidence of tendon fasciculation and crimp development by embryonic day 10; and completion of the basic cytoarchitecture of tendon observed at day 14 of embryogenesis. Collagen deposition in tendon involves first a longitudinal and then a lateral organization of tendon fibroblasts. Associated with the progressive anatomical development of tendon was an increase in birefringence retardation, mean collagen fiber profile width and a decrease in the cell volume fraction. Birefringence retardation per unit thickness did not change. The fibril packing density of the fibers remained constant, although the fibers were observed to increase in size. Apparently, collagen fibrillogenesis in vivo can be quantitatively studied by measurement of the birefringence retardation using polarized light.