Genetic basis of stage-specific melanism: a putative role for a cysteine sulfinic acid decarboxylase in insect pigmentation
Genetic basis of stage-specific melanism: a putative role for a cysteine sulfinic acid decarboxylase in insect pigmentation
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DOI：
10.1038/hdy.2011.127
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发表时间：
2012-06-01
期刊：
影响因子：
3.8
通讯作者：
Beldade, P.
中科院分区：
文献类型：
--
作者：
Saenko, S. V.;Jeronimo, M. A.;Beldade, P.
黑化，即身体整体变黑，是动物适应特定环境的一种普遍形式，包括自然选择进化的典型例子，比如胡椒蛾的工业黑化。黑色素生物合成途径的主要成分已经在模式昆虫中得到了表征，但对某些鳞翅目物种中特定生命阶段黑色素病的遗传基础知之甚少。在这里，我们研究了双环蝶的两种黑色素突变，称为巧克力和黑色素，分别只影响幼虫和成虫阶段的色素沉着。我们对孟德尔分离模式的分析表明，幼虫和成虫的黑化表型是由不同的、独立分离位点的等位基因引起的。我们的连锁定位分析排除了色素沉着候选基因black作为黑色素位点，并暗示编码推定的吡哆醛磷酸依赖半胱氨酸亚磺酸脱羧酶的基因作为巧克力位点。我们在编码序列和表达水平上显示了这种候选幼虫黑化位点的变化。这是第一个表明这种基因在昆虫中具有生物学功能的研究。我们的发现为研究该基因座在色素沉着适应性变异进化中的作用，以及在不同生态环境和身体颜色压力下发育阶段色素生物合成调节的解耦性提供了令人兴奋的机会。遗传（2012）108,594-601；doi: 10.1038 / hdy.2011.127;2012年1月11日在线发布
Melanism, the overall darkening of the body, is a widespread form of animal adaptation to particular environments, and includes bookcase examples of evolution by natural selection, such as industrial melanism in the peppered moth. The major components of the melanin biosynthesis pathway have been characterized in model insects, but little is known about the genetic basis of life-stage specific melanism such as cases described in some lepidopteran species. Here, we investigate two melanic mutations of Bicyclus anynana butterflies, called Chocolate and melanine, that exclusively affect pigmentation of the larval and adult stages, respectively. Our analysis of Mendelian segregation patterns reveals that the larval and adult melanic phenotypes are due to alleles at different, independently segregating loci. Our linkage mapping analysis excludes the pigmentation candidate gene black as the melanine locus, and implicates a gene encoding a putative pyridoxal phosphate-dependant cysteine sulfinic acid decarboxylase as the Chocolate locus. We show variation in coding sequence and in expression levels for this candidate larval melanism locus. This is the first study that suggests a biological function for this gene in insects. Our findings open up exciting opportunities to study the role of this locus in the evolution of adaptive variation in pigmentation, and the uncoupling of regulation of pigment biosynthesis across developmental stages with different ecologies and pressures on body coloration. Heredity (2012) 108, 594-601; doi:10.1038/hdy.2011.127; published online 11 January 2012