Bioactive Extract from Moringa oleifera Inhibits the Pro-inflammatory Mediators in Lipopolysaccharide Stimulated Macrophages
Bioactive Extract from Moringa oleifera Inhibits the Pro-inflammatory Mediators in Lipopolysaccharide Stimulated Macrophages
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DOI：
10.4103/0973-1298.172961
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发表时间：
2015-12-01
影响因子：
0.7
通讯作者：
Fakurazi, Sharida
中科院分区：
文献类型：
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作者：
Fard, Masoumeh Tangestani;Arulselvan, Palanisamy;Fakurazi, Sharida
炎症是一种众所周知的生理反应，可以保护身体免受感染，恢复组织损伤。然而，慢性炎症可引发各种炎症相关疾病/紊乱。辣木是一种在大多数热带国家广泛种植的植物，它在传统上被认为具有几种药用价值。目的：研究油橄榄提取物对脂多糖刺激的巨噬细胞的抗炎作用。材料与方法：通过对Griess反应中一氧化氮（NO）生成的抑制作用和巨噬细胞促炎介质的表达，评价油松氢乙醇生物活性叶提取物的抗炎作用。结果：有趣的是，我们发现油松生物活性叶提取物显著抑制NO生成和其他炎症标志物的分泌，如前列腺素E-2、肿瘤坏死因子α、白细胞介素(IL)-6和IL-1 β。同时，生物活性提取物诱导MO的产生呈剂量依赖性。此外，油桐氢乙醇生物活性叶提取物有效抑制lps诱导的RAW264.7巨噬细胞炎症标志物诱导的NO合成酶、环氧合酶-2和活化b细胞p65的核因子kappa-轻链增强子的蛋白表达，并呈剂量依赖性。结论：这些发现支持了油橄榄植物作为炎症相关疾病/紊乱的有效治疗方法的传统应用。
Introduction: Inflammation is a well-known physiological response to protect the body against infection and restore tissue injury. Nevertheless, the chronic inflammation can trigger various inflammatory associated diseases/disorder. Moringa oleifera is a widely grown plant in most tropical countries and it has been recognized traditionally for several medicinal benefits. Objectives: The objective of this study was to investigate the anti-inflammatory properties of M. oleifera extract on lipopolysaccharide (LPS)- stimulated macrophages. Materials and Methods: The anti-inflammatory effect of M. oleifera hydroethanolic bioactive leaves extracts was evaluated by assessing the inhibition of nitric oxide (NO) production during Griess reaction and the expression of pro-inflammatory mediators in macrophages. Results: Interestingly, we found that M. oleifera hydroethanolic bioactive leaves extract significantly inhibited the secretion of NO production and other inflammatory markers such as prostaglandin E-2, tumor necrosis factor alpha, interleukin (IL)-6, and IL-1 beta. Meanwhile, the bioactive extract has induced the production of MO in a dose-dependent manner. In addition, M. oleifera hydroethanolic bioactive leaves extract effectively suppressed the protein expression of inflammatory markers inducible NO synthase, cyclooxygenase-2, and nuclear factor kappa-light-chain-enhancer of activated B-cells p65 in LPS-induced RAW264.7 macrophages in a dose-dependent manner. Conclusion: These findings support the traditional use of M. oleifera plant as an effective treatment for inflammation associated diseases/disorders.