CD44 and hyaluronan-dependent rolling interactions of lymphocytes on tonsillar stroma.
CD44 and hyaluronan-dependent rolling interactions of lymphocytes on tonsillar stroma.
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DOI：
10.1083/jcb.134.4.1075
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发表时间：
1996-08
期刊：
影响因子：
--
通讯作者：
Springer TA
中科院分区：
文献类型：
--
作者：
Clark RA;Alon R;Springer TA
关于淋巴细胞如何在次级淋巴器官内迁移知之甚少。基质细胞及其相关的网状纤维形成纤维网络，从高内皮微静脉辐射到淋巴结的所有区域，并可为淋巴细胞迁移提供支架。我们研究了淋巴细胞与培养的人扁桃体基质细胞及其细胞外基质的相互作用，使用剪切力来区分短暂的相互作用和牢固的粘附。扁桃体淋巴细胞和SKW 3 T淋巴瘤细胞在培养的扁桃体基质细胞及其基质的单层上栓系和滚动。这些滚动相互作用的一个显着比例是独立的二价阳离子和介导的CD 44结合透明质酸，如所示的抑制与单克隆抗体CD 44，可溶性透明质酸，透明质酸酶处理的基板，和O-糖蛋白酶处理的滚动细胞。O-糖蛋白酶处理的基板也完全阻断结合基质基质和部分基质单层。SKW 3细胞在塑料固定的透明质酸上栓系和滚动，证实了这种相互作用的特异性。相反，单层的静息或刺激的人脐静脉内皮细胞不能支持CD 44和透明质酸依赖的滚动。在流动条件下，将SKW 3细胞加入人扁桃体的冷冻切片中，在EDTA存在下沿网状纤维沿着卷曲。滚动被CD 44 mAb或透明质酸阻断。我们认为，淋巴细胞迁移通过次级淋巴器官可能使用CD 44结合透明质酸固定在基质细胞和网状纤维。
Little is known about how lymphocytes migrate within secondary lymphoid organs. Stromal cells and their associated reticular fibers form a network of fibers that radiate from high endothelial venules to all areas of the lymph node and may provide a scaffold for lymphocyte migration. We studied interactions of lymphocytes with cultured human tonsillar stromal cells and their extracellular matrix using shear stress to distinguish transient interactions from firm adhesion. Tonsillar lymphocytes and SKW3 T lymphoma cells tethered and rolled on monolayers of cultured tonsillar stromal cells and their matrix. A significant proportion of these rolling interactions were independent of divalent cations and were mediated by CD44 binding to hyaluronan, as shown by inhibition with mAb to CD44, soluble hyaluronan, as hyaluronidase treatment of the substrate, and O-glycoprotease treatment of the rolling cells. O-glycoprotease treatment of the substrate also blocked binding completely to stromal matrix and partially to stromal monolayers. SKW3 cells tethered and rolled on plastic-immobilized hyaluronan, confirming the specificity of this interaction. By contrast, monolayers of resting or stimulated human umbilical vein endothelial cells failed to support CD44- and hyaluronan-dependent rolling. SKW3 cells added under flow conditions to frozen sections of human tonsil bound and rolled along reticular fibers in the presence of EDTA. Rolling was blocked by either CD44 mAb or hyaluronan. We propose that lymphocytes migrating through secondary lymphoid organs may use CD44 to bind to hyaluronan immobilized on stromal cells and reticular fibers.