Prepartum measurement of serum biomarkers reflecting osteoclastic and osteoblastic bone metabolism for predicting the risk of milk fever in dairy cows
Prepartum measurement of serum biomarkers reflecting osteoclastic and osteoblastic bone metabolism for predicting the risk of milk fever in dairy cows
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DOI：
10.1017/s0022029922000218
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发表时间：
2022-03-15
影响因子：
2.1
通讯作者：
Kawashima，Chiho
中科院分区：
文献类型：
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作者：
Yamagishi，Norio;Kawashima，Chiho
我们研究了产前血清骨生物标志物水平是否与胎次提前的奶牛的产时低钙血症程度和产奶热 (MF) 风险相关。总共 58 头妊娠晚期牛被分为 4 组：未产组、初产组、第 2 次泌乳期经产组和第 3-5 次泌乳期经产期。根据 MF 的发生情况，将经产奶牛进一步分为 MF 组和非 MF 组。在产前 3 周至产后 5 天期间从奶牛身上采集血清样本，用于测量血清钙 (Ca) 和三种骨生物标志物：抗酒石酸酸性磷酸酶同工型 5b (TRAP5b)、骨保护素 (OPG) 和碱性磷酸酶骨同工酶 (ALP3)。计算OPG与TRAP5b的比率（O/T比率）和ALP3与TRAP5b的比率（A/T比率）。所有牛的数据显示，分娩时低钙血症的严重程度随着胎次/日龄的增加而增加。 MF 奶牛分娩后血清 TRAP5b 活性升高，O/T 比值降低，表明破骨细胞生成导致破骨细胞数量增加，以应对严重的低钙血症。 MF 奶牛在产前 3 周内的血清 ALP3 活性低于非 MF 奶牛，因此，MF 奶牛产前成骨细胞功能可能较弱。在产前 2-3 周内，经产奶牛血清 ALP3 活性和 A/T 比值与第 0 天（产犊日）血清 Ca 浓度呈中度相关，受试者工作特征曲线分析显示 ALP3 活性具有良好的预测 MF 的能力。总之，产前血清 ALP3 活性是预测经产奶牛 MF 的有前途的生物标志物。
We investigated whether prepartum levels of serum bone biomarkers are related to the degree of parturient hypocalcaemia and risk of milk fever (MF) in dairy cows with advancing parity. A total of 58 late-pregnant cattle were assigned to four groups: nulliparous, primiparous, multiparous in the 2nd lactation and multiparous in the 3rd–5th lactation. The multiparous cows were further assigned to MF and non-MF groups according to the onset of MF. Serum samples were obtained from the cows during the 3 weeks prepartum to 5 d postpartum period for the measurement of serum calcium (Ca) and three bone biomarkers: tartrate-resistant acid phosphatase isoform 5b (TRAP5b), osteoprotegerin (OPG) and bone isoenzyme of alkaline phosphatase (ALP3). The ratios of OPG to TRAP5b (O/T ratio) and ALP3 to TRAP5b (A/T ratio) were calculated. The data from all cattle showed that the severity of hypocalcaemia at parturition increased with advancing parity/age. The MF cows had elevated serum TRAP5b activity and a decreased O/T ratio after parturition, suggesting an increased number of osteoclasts due to osteoclastogenesis, in response to severe hypocalcaemia. The MF cows showed lower serum ALP3 activity during the 3 weeks prepartum than the non-MF cows, therefore, prepartum osteoblast function was likely weak in the MF cows. During the 2–3 weeks prepartum, serum ALP3 activity and the A/T ratio had moderate associations with the serum Ca concentration at day 0 (day of calving) in the multiparous cows, and receiver operating characteristic curve analysis revealed that ALP3 activity had excellent ability to predict MF. In conclusion, prepartum serum ALP3 activity is a promising biomarker to predict MF in multiparous cows.