Nonsymmetrical Dynamics of the HBV Capsid Assembly and Disassembly Evidenced by Their Transient Species.
Nonsymmetrical Dynamics of the HBV Capsid Assembly and Disassembly Evidenced by Their Transient Species.
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DOI：
10.1021/acs.jpcb.0c05024
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发表时间：
2020-11-12
期刊：
影响因子：
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通讯作者：
Tresset, Guillaume
中科院分区：
文献类型：
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作者：
Chevreuil, Maelenn;Lecoq, Lauriane;Tresset, Guillaume
与生物物理学中研究的许多蛋白质多聚体一样，B型肝炎病毒（HBV）衣壳蛋白的组装和拆卸动力学途径是不对称的。利用时间分辨的小角X射线散射和奇异值分解分析，我们已经调查了这些过程在体外的盐度或离液性的快速变化。沿着组装途径，经典的成核-生长机制之后是缓慢的弛豫阶段，在此期间，根据理论预测，最后几个亚基的捕获是缓慢的，类似于captain的瞬时物种自组织。相比之下，分解过程通过意想不到的、分形分支的亚基簇进行，这些亚基簇最终在更长的时间尺度上消失。一方面，我们的研究结果证实并扩展了以前的观点，观察到的滞后现象和理论在衣壳的形成和解离。另一方面，他们发现了可能与HBV亚单位在病毒周期中的功能直接相关的细节。
As with many protein multimers studied in biophysics, the assembly and disassembly dynamical pathways of hepatitis B virus (HBV) capsid proteins are not symmetrical. Using time-resolved small-angle X-ray scattering and singular value decomposition analysis, we have investigated these processes in vitro by a rapid change of salinity or chaotropicity. Along the assembly pathway, the classical nucleation-growth mechanism is followed by a slow relaxation phase during which capsid-like transient species self-organize in accordance with the theoretical prediction that the capture of the few last subunits is slow. By contrast, the disassembly proceeds through unexpected, fractal-branched clusters of subunits that eventually vanish over a much longer time scale. On the one hand, our findings confirm and extend previous views as to the hysteresis phenomena observed and theorized in capsid formation and dissociation. On the other hand, they uncover specifics that may directly relate to the functions of HBV subunits in the viral cycle.