A quantitative autoradiographic study of 125I atrial natriuretic factor in the heart of a teleost fish (Conger conger).
A quantitative autoradiographic study of 125I atrial natriuretic factor in the heart of a teleost fish (Conger conger).
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硬骨鱼 (Conger conger) 心脏 125I 心房钠尿因子的定量放射自显影研究。
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
Bruno Tota
中科院分区：
文献类型：
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作者：
M. Cerra;Marcello Canonaco;Bruno Tota
定量受体放射自显影研究显示，125i -心房利钠肽因子（ANF）结合在不同心脏区域存在异质分布。ANF在动脉球茎最内层（内膜）的结合密度升高（3790 fmol/mg蛋白），而在心房和心室的结合密度降低（293-403 fmol/mg蛋白）。为了确定125I-ANF在上述心脏部位的结合特性（KD, Bmax），进行了饱和结合试验。结果显示，相对于心室较高的125I-ANF KD值（373 pM），心房和动脉球部的125I-ANF KD值较低（28.8-52.6 pM）。心房和心室的结合位点数量分别为632和1279 fmol/mg蛋白，而球动脉的Bmax显著升高，为7235 fmol/mg蛋白。这些结果可能对ANF受体结合活性及其对不同心功能的调节作用提供一些见解。
Quantitative receptor autoradiographic study of 125I-atrial natriuretic peptide factor (ANF) in the heart of a teleost fish Conger conger has shown that a heterogenous distribution of 125I-ANF binding exists in the different cardiac regions. Elevated ANF binding densities (3,790 fmol/mg protein) were encountered in the innermost layer (tunica intima) of the bulbus arteriosus while lower binding levels (293-403 fmol/mg protein) were revealed in atrium and ventricle. In order to determine 125I-ANF binding characteristics (KD, Bmax) in the above cardiac sites, saturation binding assays were carried out. The results show that low 125I-ANF KD values (28.8-52.6 pM) were found in the atrium and in the bulbus arteriosus with respect to the higher KD values (373 pM) of the ventricle. The number of binding sites were respectively 632 and 1,279 fmol/mg protein for the atrium and the ventricle, while a substantially elevated Bmax of 7,235 fmol/mg protein was found for the bulbus arteriosus. These results may furnish some insights concerning ANF receptor binding activity and its putative regulatory role of different cardiac functions.