NATURE OF COMPETENCE-INDUCING FACTOR IN BACILLUS SUBTILIS
NATURE OF COMPETENCE-INDUCING FACTOR IN BACILLUS SUBTILIS
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DOI：
10.1016/0006-291x(67)90090-3
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发表时间：
1967-01-01
影响因子：
3.1
通讯作者：
BARKER, GR
中科院分区：
文献类型：
--
作者：
AKRIGG, A;AYAD, SR;BARKER, GR
结果和讨论从表I可以看出，在用最低限度的培养基洗涤后，枯草杆菌1681-感受态细胞的转化能力没有明显下降。然而，用蒸馏水洗涤将转化子的数量减少到可以忽略不计的价值。将水提物添加到水洗过的细胞中，恢复了它们转化的能力。这些结果表明，释放到水提物中的一个因子对于保留枯草杆菌1681-的活性是必不可少的。这些结果类似于Parijskaya和P&L-Ova(1967)所描述的结果。在本实验中，对能力诱导活性进行了部分纯化，并对相关材料的性质进行了研究。
RESULTS AND DISCUSSION From Table I, it can be seen that after being washed with minimal medium, competent cells of _B. subtilis 1681-suffered no significant reduction in their ability to be transformed. However, washing with distilled water reduced the number of transformants to a negligible value. Addition of the aqueous extract to the waterwashed cells restored their ability to be transformed. These results indicated that a factor which is released into the aqueous extract is essential for the retention of competence ins subtilis 1681-. These results are similar to those described by Parijskaya and P&l-ova(1967). In the present experiments, the competence inducing activity has been partially purified and the nature of the material responsible has been investigated.