Vertical niche differentiation of ectomycorrhizal hyphae in soil as shown by T-RFLP analysis
Vertical niche differentiation of ectomycorrhizal hyphae in soil as shown by T-RFLP analysis
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DOI：
10.1046/j.1469-8137.2002.00535.x
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发表时间：
2002-12-01
期刊：
影响因子：
9.4
通讯作者：
Koide, RT
中科院分区：
文献类型：
--
作者：
Dickie, IA;Xu, B;Koide, RT
不同土壤基质的生态位分化被认为是外生菌根真菌多样性的一种机制。本研究采用土壤DNA提取、PCR和末端限制性片段长度多态性（T-RFLP）分析相结合的方法，对森林地表4个不同层次真菌菌丝的垂直分布进行了研究结果表明，不同层次（下层、F层、H层和B层）的真菌群落结构差异显著。聚类分析表明，6种不同的资源利用模式：凋落物层专家，凋落物层通才，F层，H层，B层物种，和多层通才。已知的外生菌根物种都被发现在所有的六个集群。这种观察外生菌根真菌之间的空间分配沿着一个单一的，相对简单的基质资源梯度支持生态位分化假说作为一个重要的机制，有助于外生菌根真菌多样性。
Niche differentiation for different soil substrates has been proposed as a mechanism contributing to ectomycorrhizal fungal diversity. This hypothesis has been largely untestable because of a lack of techniques to study the in situ distribution of ectomycorrhizal hyphae.We developed a technique involving soil DNA extraction, PCR and terminal restriction fragment length polymorphism (T-RFLP) analysis for species identification to investigate the vertical distribution of fungal hyphae in four distinct layers of the forest floor (lower litter, F-layer, H-layer, and B-horizon) of a Pinus resinosa plantation.Fungal communities differed markedly among the four layers. Cluster analysis suggested six different patterns of resource utilization: litter-layer specialists, litter-layer generalists, F-layer, H-layer, and B-horizon species, and multilayer generalists. Known ectomycorrhizal species were found in all six clusters.This spatial partitioning observed among ectomycorrhizal fungi along a single, relatively simple substrate-resource gradient supports the niche differentiation hypothesis as an important mechanism contributing to ectomycorrhizal fungal diversity.