Comparison of the tetrazolium salt assay for succinate dehydrogenase with the cytosensor microphysiometer in the assessment of compound toxicities
Comparison of the tetrazolium salt assay for succinate dehydrogenase with the cytosensor microphysiometer in the assessment of compound toxicities
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DOI：
10.1006/abio.1999.4274
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发表时间：
1999-10-15
影响因子：
2.9
通讯作者：
O'Kennedy, R
中科院分区：
文献类型：
--
作者：
Cooke, D;O'Kennedy, R
将细胞传感器微生理计（用于检测细胞代谢的生物传感仪器）与作为化学敏感性测试的已建立的四唑盐测定进行比较。两个香豆素化合物，7-羟基香豆素和escuoke，进行了检查，以确定其对A431细胞的细胞代谢的影响超过24小时的曝光期。在四唑盐测定中，7-羟基香豆素在浓度大于10 μ g/ml时引起琥珀酸脱氢酶活性的抑制。Escuoke对琥珀酸脱氢酶的影响更严重，在大于1 μ g/ml时观察到活性降低。对于检查的两种化合物，观察到的效应均具有剂量依赖性。暴露于100 μ g/ml药物的细胞的代谢活性分别为对照值的90.37 +/- 2.8和71.62 +/- 2.96（n = 3），对于7-羟基香豆素和七叶苷。使用cytosensor microphysiometer来评估代谢活动，观察到类似的抑制模式，escuoke比7-羟基香豆素对细胞代谢更有害。两种化合物的作用均具有剂量和时间依赖性。7-羟基香豆素（100 μ g/ml）使细胞代谢率降至对照代谢率的44.21 ± 5.34%（n = 4），而100 μ g/ml七叶酮使代谢率降至对照代谢率的21.5 ± 4.54%（n = 4）。细胞传感器方法被证明是上级的四唑盐测定的一些原因，这在本文中进行了讨论。(C)北京：科学出版社.
The cytosensor microphysiometer (a biosensing instrument for detecting cellular metabolism) was compared to the established tetrazolium salt assay as a chemosensitivity test. Two coumarin compounds, 7-hydroxycoumarin and esculetin, were examined to determine their effect on the cellular metabolism of A431 cells over a 24-h exposure period. In the tetrazolium salt assay, 7-hydroxycoumarin caused suppression of the succinate dehydrogenase activity at concentrations greater than 10 mu g/ml. Esculetin exerted a more serious effect on succinate dehydrogenase, with decreases in activity observed at greater than 1 mu g/ml. The observed effect was dose-dependent for both compounds examined. The metabolic activities of cells exposed to 100 mu g/ml of drug were 90.37 +/- 2.8 and 71.62 +/- 2.96 (n = 3), of control values, for 7-hydroxycoumarin and esculetin, respectively. Using the cytosensor microphysiometer to assess metabolic activities, a similar pattern of inhibition was observed, with esculetin more detrimental to cellular metabolism than 7-hydroxycoumarin. The effect was dose-a and time-dependent for both compounds. 7-Hydroxycoumarin (100 mu g/ml) caused the cellular metabolic rate to drop to 44.21 +/- 5.34% (n = 4) of the control metabolic rate, while 100 mu g/ml esculetin caused the metabolic rate to fall to 21.5 +/- 4.54% (n = 4) of the control rate. The cytosensor method proved to be superior to the tetrazolium salt assay for a number of reasons, which are discussed in this paper. (C) 1999 Academic Press.