An improved method for discrimination of cell populations in tissue sections using microscopy-based multicolor tissue cytometry
An improved method for discrimination of cell populations in tissue sections using microscopy-based multicolor tissue cytometry
复制标题
DOI：
10.1002/cyto.a.20219
复制
发表时间：
2006-03-01
期刊：
影响因子：
3.7
通讯作者：
Steiner, GE
中科院分区：
文献类型：
--
作者：
Ecker, RC;Rogojanu, R;Steiner, GE
背景：在组织学背景下，研究人员和病理学家缺乏一个普遍适用的细胞学参数定量测定标准。随着对疾病特异性标志物认识的增加，需要一种合适的原位组织细胞术。方法：基于显微镜的多色组织细胞术(MMTC)可以对组织中的单个细胞进行多色分析。结果：对CD45/CD3/CD4/CD8染色的组织标本进行了分析。MMTC的特异性和重复性良好，并介绍了一种新的基于MMTC的功能，以提高亚群的视觉辨别能力。结论：我们的数据表明，MMTC是实现固体组织和细胞单层荧光定量自动化的重要一步。(C)2006年国际分析细胞学学会。
Background: In tissue context, researchers and pathologists lack a generally applicable standard for quantitative determination of cytological parameters. Increasing knowledge of disease-specific markers calls for an appropriate in situ tissue cytometry.Methods: Microscopy-based multicolor tissue cytometry (MMTC) permits multicolor analysis of single cells within tissue context.Results: Tissue specimens stained for CD45/CD3/CD4/CD8 were analyzed. Specificity,is well,is reproducibility of MMTC is demonstrated and a novel MMTC-based function to improve Visual discrimination of subpopulations is introduced.Conclusions: Our data demonstrate that MMTC constitutes an important step toward automated and quantitative fluorometry of solid tissues and cell monolayers. (c) 2006 International Society for Analytical Cytology.