Standardized Histomorphometric Evaluation of Osteoarthritis in a Surgical Mouse Model.
Standardized Histomorphometric Evaluation of Osteoarthritis in a Surgical Mouse Model.
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DOI：
10.3791/60991
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发表时间：
2020-05-06
期刊：
影响因子：
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通讯作者：
Kamal F
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文献类型：
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作者：
Pinamont WJ;Yoshioka NK;Young GM;Karuppagounder V;Carlson EL;Ahmad A;Elbarbary R;Kamal F
骨关节炎（OA）是美国最常见的关节疾病之一，其特征在于关节软骨（主要是髋关节和膝关节）的进行性变性，这对患者的活动性和生活质量产生了重大影响。到目前为止，还没有能够减缓或抑制软骨退化的现有OA治愈性疗法。目前，正在进行广泛的研究，以了解OA病理学，并发现新的治疗方法或药物，可以有效地减缓，停止，甚至逆转OA。因此，至关重要的是要有一个定量的和可重复的方法，以准确地评估OA相关的关节软骨，滑膜和软骨下骨的病理变化。目前，OA严重程度和进展主要使用国际骨关节炎研究协会（OARSI）或Mankin评分系统进行评估。尽管这些评分系统很重要，但它们是半定量的，可能会受到用户主观性的影响。更重要的是，它们不能准确地评估早期疾病状态或早期治疗阶段软骨的细微但重要的变化。我们在这里描述的协议使用计算机化和半自动化的组织形态学软件系统，以建立一个标准化的，严格的，可重复的定量方法来评估关节OA的变化。该协议是对现有系统的有力补充，并允许更有效地检测关节中的病理变化。
One of the most prevalent joint disorders in the United States, osteoarthritis (OA) is characterized by progressive degeneration of articular cartilage, primarily in the hip and knee joints, which results in significant impacts on patient mobility and quality of life. To date, there are no existing curative therapies for OA able to slow down or inhibit cartilage degeneration. Presently, there is an extensive body of ongoing research to understand OA pathology and discover novel therapeutic approaches or agents that can efficiently slow down, stop, or even reverse OA. Thus, it is crucial to have a quantitative and reproducible approach to accurately evaluate OA-associated pathological changes in the joint cartilage, synovium, and subchondral bone. Currently, OA severity and progression are primarily assessed using the Osteoarthritis Research Society International (OARSI) or Mankin scoring systems. In spite of the importance of these scoring systems, they are semiquantitative and can be influenced by user subjectivity. More importantly, they fail to accurately evaluate subtle, yet important, changes in the cartilage during the early disease states or early treatment phases. The protocol we describe here uses a computerized and semiautomated histomorphometric software system to establish a standardized, rigorous, and reproducible quantitative methodology for the evaluation of joint changes in OA. This protocol presents a powerful addition to the existing systems and allows for more efficient detection of pathological changes in the joint.