Evaluation of chemical treatment combined with vacuum and ultrasonication with a water resonance system for reducing Campylobacter on naturally contaminated chicken carcasses
Evaluation of chemical treatment combined with vacuum and ultrasonication with a water resonance system for reducing Campylobacter on naturally contaminated chicken carcasses
复制标题
DOI：
10.1016/j.foodcont.2020.107087
复制
发表时间：
2020-06-01
期刊：
影响因子：
6
通讯作者：
Misawa, Naoaki
中科院分区：
文献类型：
--
作者：
Vetchapitak, Torrung;Shinki, Taisuke;Misawa, Naoaki
本研究旨在评估一种新技术去除自然污染的散养鸡和肉鸡尸体中弯曲杆菌的效果。将从商业加工厂取出内脏后获得的尸体浸入0.1%氯化十六烷基吡啶（CPC）或0.01%次氯酸钠（NaOCl）中，并暴露于-0.02 MPa的真空中以从羽毛中除去空气。然后将尸体浸入钢容器中的消毒剂溶液中，并使用水共振系统（WRS）在130 kHz下进行超声处理。还使用自来水代替消毒剂，其他尸体单独浸入每种化学品中。乳房和背部皮肤上的弯曲杆菌计数采用最大可能数（MPN）法确定。这种使用CPC和NaOCl的新治疗显著减少了背部和乳房皮肤上的弯曲杆菌计数。用0.1%CPC结合WRS真空和超声波处理是最有效的，在自由放养和肉鸡尸体上分别实现弯曲杆菌减少1.36-1.64 log（10）MPN/10 g和0.94-1.16 log（10）MPN/10 g。扫描电子显微镜（SEM）证实，与未经处理的皮肤相比，该处理成功地从乳房皮肤上去除了碎屑。这些结果表明，采用真空和超声处理与WRS的组合的消毒剂的使用对于减少鸡尸体上的弯曲杆菌的计数是非常有效的。
The present study was conducted to evaluate the efficacy of a new technique for removal of campylobacters from naturally contaminated free-range and broiler chicken carcasses. The carcasses, obtained from commercial processing plants after evisceration, were immersed in 0.1% cetylpyridinium chloride (CPC) or 0.01% sodium hypochlorite (NaOCl) and exposed to a vacuum of -0.02 MPa to remove air from feather. The carcasses were then immersed in a disinfectant solution in a steel container and subjected to ultrasonication with a water resonance system (WRS) at 130 kHz. Tap water was also used instead of the disinfectant, and other carcasses were immersed in each chemical alone. The Campylobacter count on breast and back skin was determined by the most-probable-number (MPN) method. This new treatment using CPC and NaOCl significantly reduced the Campylobacter count on back and breast skin. Treatment with 0.1% CPC combined with vacuum and ultrasonication with the WRS was the most effective, achieving a Campylobacter reduction of 1.36-1.64 log(10) MPN/10 g and 0.94-1.16 log(10) MPN/10 g on free-range and broiler chicken carcasses, respectively. Scanning electron microscopy (SEM) confirmed that this treatment successfully removed debris from breast skin in comparison to untreated skin. These results suggest that the use of sanitizers employing a combination of vacuum and ultrasonication with a WRS is highly effective for reducing the count of campylobacters on chicken carcasses.