Quantitative analysis of untreated bio-samples
Quantitative analysis of untreated bio-samples
复制标题
DOI：
10.1016/s0168-583x(98)01071-4
复制
发表时间：
1999-04-01
影响因子：
1.3
通讯作者：
Matsuda, K
中科院分区：
文献类型：
--
作者：
Sera, K;Futatsugawa, S;Matsuda, K
开发了一种对未处理样品进行定量分析的无标样方法，对于头发样品，直接用质子束照射几根头发进行测量，并通过我们自己开发的无标样方法进行定量分析，首先导出锌浓度的定量值，然后以锌为内标获得其他元素的浓度。结果。 40个样品的硫浓度值与典型日本人的平均值非常吻合，并且彼此之间的吻合度在20%以内，可以证实本方法的有效性。通过将结果与通常的内标法获得的结果进行比较来确认准确性。为了对骨样本进行表面分析，使用了非常小的质子束入射角，因此射弹的能量损失和 X 射线的自吸收都可以忽略不计。结果，通过无标样方法获得了许多元素的一致浓度值。 (C) 1999 Elsevier Science B.V. 保留所有权利。
A standard-free method of quantitative analysis for untreated samples has been developed, For hair samples, measurements were performed by irradiating with a proton beam a few hairs as they are, and quantitative analysis was carried out by means of a standard-free method developed by ourselves, First, quantitative values of concentration of zinc were derived, then concentration of other elements was obtained by regarding zinc as an internal standard. As the result. values of concentration of sulphur for 40 samples agree well with the average value for a typical Japanese and also with each other within 20%, and validity of the present method could be confirmed, Accuracy was confirmed by comparing the results with those obtained by the usual internal standard method, too. For the purpose of a surface analysis of a bone sample, a very small incidence angle of the proton beam was used, so that both energy loss of the projectile and self-absorption of X-rays become negligible. As the result, consistent values of concentration for many elements were obtained by the standard-free method. (C) 1999 Elsevier Science B.V. All rights reserved.