Sensitive determination of tenofovir in human plasma samples using reversed-phase liquid chromatography
Sensitive determination of tenofovir in human plasma samples using reversed-phase liquid chromatography
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DOI：
10.1016/s1570-0232(03)00333-7
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发表时间：
2003-08-15
影响因子：
3
通讯作者：
Aymard, G
中科院分区：
文献类型：
--
作者：
Sentenac, S;Fernandez, C;Aymard, G
建立了测定人血浆中核苷酸类似物替诺福韦浓度的高效液相色谱方法。固-液萃取法与反相高效液相色谱联用。该系统需要Na2HPO4缓冲液、四丁基硫酸氢铵和乙腈组成的流动相，通过C-18柱进行不同的洗脱。方法的准确度、精密度和线性范围为10~4000 ng/ml，已用于11例HIV感染者多次抗逆转录病毒治疗后病毒学失败患者的血药浓度测定。该方法还成功地应用于一例HIV感染的肾功能衰竭患者的药代动力学研究。(C)2003爱思唯尔B.V.保留所有权利。
A new high-performance liquid chromatography assay was developed for the determination of tenofovir, a nucleotide analogue, in plasma. A solid-liquid extraction procedure was coupled with a reversed-phase HPLC system. The system requires a mobile phase containing Na2HPO4 buffer, tetrabutylammonium hydrogen sulfate and acetonitrile for different elution through a C-18 column with UV detection. The method proved to be accurate, precise and linear between 10 and 4000 ng/ml. The method was applied to determine trough levels of tenofovir in 11 HIV-infected patients with virologic failure under multiple antiretroviral therapy. This method was also successfully applied to a pharmacokinetic study in an HIV infected patient with renal failure. (C) 2003 Elsevier B.V. All rights reserved.