AMINO-ACID-SEQUENCE OF A LIGHT CHAIN VARIABLE REGION OF A HUMAN RHEUMATOID-FACTOR OF THE WA IDIOTYPIC GROUP, IN PART PREDICTED BY ITS REACTIVITY WITH ANTIPEPTIDE ANTIBODIES
AMINO-ACID-SEQUENCE OF A LIGHT CHAIN VARIABLE REGION OF A HUMAN RHEUMATOID-FACTOR OF THE WA IDIOTYPIC GROUP, IN PART PREDICTED BY ITS REACTIVITY WITH ANTIPEPTIDE ANTIBODIES
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DOI：
10.1016/0161-5890(86)90049-0
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发表时间：
1986-03-01
影响因子：
3.6
通讯作者：
CAPRA, JD
中科院分区：
文献类型：
--
作者：
NEWKIRK, M;CHEN, PP;CAPRA, JD
将抗肽抗血清培养至来自人单克隆IgM（Sie）的轻链的第二和第三互补决定区，该IgM具有抗丙种球蛋白活性并属于人类风湿因子的Wa交叉反应独特型组，其中两个成员（Sie，Wol）先前已在我们的实验室中测序（Andrews和Capra，Biochemistry 20，5816-5822，1981）。发现这些抗血清与共享Wa独特型的另一种人单克隆IgM（Gol）的轻链反应，而针对Sie重链的第三CDR制备的抗肽抗血清未能反应。发现Gol轻链可变区的氨基酸序列与合成肽来源的Sie轻链高度同源，特别是在框架区和第二和第三CDR中。这项研究阐明，抗肽抗血清是有价值的和特异性的探针，用于确定分子之间的关系，表现出类似的抗原结合或独特型特异性，而且，这种抗血清能够预测氨基酸序列与令人惊讶的精度。
Antipeptide antisera were raised to the second and third complementary-determining regions of the light chain derived from a human monoclonal IgM (Sie) which had antigammaglobulin activity and belonged to the Wa cross-reactive idiotypic group of human rheumatoid factors, two of whose members (Sie, Wol) had been previously sequenced in our laboratory (Andrews and Capra, Biochemistry 20, 5816-5822, 1981). These antisera were found to react with the light chain of another human monoclonal IgM (Gol) that shared the Wa idiotype while antipeptide antisera made to the third CDR of the Sie heavy chain failed to react. The amino acid sequence of the variable region of the Gol light chain was found to be highly homologous to the light chain of Sie from which the synthetic peptides were derived, particularly in the framework regions and the second and third CDR. This study illustates that antipeptide antisera are valuable and specific probes for determining the relationship between molecules which exhibit similar antigen binding or idiotypic specificities and, furthermore, such antisera are able to predict amino acid sequences with surprising precision.