Immunoassay of thyroxine in unextracted human serum.
Immunoassay of thyroxine in unextracted human serum.
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未提取的人血清中甲状腺素的免疫测定。
DOI：
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发表时间：
1973
影响因子：
5.8
通讯作者：
F. Wu
中科院分区：
文献类型：
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作者：
P. Larsen;J. Dočkalová;D. Sipula;F. Wu
摘要 提出了一种未提取人血清中甲状腺素 (T4) 的免疫测定方法。通过用牛甲状腺球蛋白免疫兔子获得甲状腺素结合抗体。水杨酸钠用于阻断标记和未标记的 T4 与血清甲状腺素结合蛋白的结合。通过葡聚糖包被的木炭的吸附来分离结合的和游离的标记激素。该方法足够灵敏，可以使用 20 μ1 血清进行两次稀释的重复测定。该方法的精度与竞争性蛋白结合测定相当，并且在所有临床状态下，这两种方法对 T4 的估计之间具有良好的相关性（相关系数为 0.97）。
ABSTRACT A method for the immunoassay of thyroxine (T4) in unextracted human serum is presented. Thyroxine-binding antibody was obtained by immunization of a rabbit with bovine thyroglobulin. Sodium salicylate is used to block the binding of both labeled and unlabeled T4 to serum thyroxine-binding proteins. Bound and free labeled hormone are separated by adsorption to dextran-coated charcoal. The method is sufficiently sensitive to allow duplicate determinations at two dilutions using 20 μ1 of serum. The precision of the method is comparable to the competitive protein binding assay and there is excellent correlation between the estimation of T4 by these two methods (correlation coefficient, 0.97) in all clinical states.