In vivo incorporation of an alkyne into proteins in Escherichia coli
In vivo incorporation of an alkyne into proteins in Escherichia coli
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DOI：
10.1016/j.bmcl.2004.12.065
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发表时间：
2005-03-01
影响因子：
2.7
通讯作者：
Schultz, PG
中科院分区：
文献类型：
--
作者：
Deiters, A;Schultz, PG
通过遗传选择，我们鉴定了 M.janaschii 酪氨酰-tRNA 合成酶的突变体，该突变体在大肠杆菌中选择性地用对炔丙氧基苯丙氨酸对琥珀抑制 tRNA 进行充电。这些进化的 tRNA 合成酶对用于将炔基基团特异性地整合到蛋白质中，随后在温和的反应条件下通过 [3+2]-环加成反应与荧光染料缀合。 (c) 2004 Elsevier Ltd. 保留所有权利。
Using a genetic selection we identified mutants of the M. janaschii tyrosyl-tRNA synthetase that selectively charge an amber suppressor tRNA with para-propargyloxyphenylalanine in Escherichia coli. These evolved tRNA-synthetase pairs were used to site-specifically incorporate an alkynyl group into a protein, which was subsequently conjugated with fluorescent dyes by a [3+2]-cycloaddition reaction under mild reaction conditions. (c) 2004 Elsevier Ltd. All rights reserved.